湖南中医药大学学报2016，Vol.36Issue(12)：1-6,6.DOI:10.3969/j.issn.1674-070X.2016.12.001
补阳还五汤对去势脑缺血雌性大鼠海马神经干细胞增殖及ERK/CREB信号通路的影响
Effects of Buyang Huanwu Decoction on Neural Stem Cells Proliferation and ERK/CREB Signaling Pathway in Hippocampus after Cerebral Ischemia in Ovariectomized Female Rats
摘要
Abstract
Objective To investigate the effects of Buyang Huanwu Decoction on neural stem cells (NSCs) proliferation and ERK/ CREB signaling pathway in hippocampus after cerebral ischemia in ovariectomized female rats. Methods The o-variectomized female rats with cerebral ischemia model was established by the methods of ovariectomy (OVX)and middle cere-bral artery occlusion (MCAO). The 36 rats were randomly divided into 6 groups, double sham-operation group, com-pound model group, estrogen group, estrogen+G15 group and Buyang Huanwu Decoction group and Buyang Huanwu Decoc tion+G15 group, 6 rats in each group. The rats were given corresponding medicine intragastric administration for 14 days from 24 hours after operation, at the same time, BrdU and G15 were intraperitoneal injected to label the proliferation cells and block GPER-1 in each day, respectively. The NSCs proliferation of ischemic hippocampal dentate gyrus in rats was detected using immunofluorescence BrdU / nestin double staining with paraffin sections, the SP method of immunohistochemistry was used to detect the expression level of ERK1/2, CREB1 phosphorylation protein. Results Compared with the compos-ite model group, the number of double positive cells and pERK1/2, pCREB1 positive cells in ischemic hippocampal DG area of Buyang Huanwu Decoction group and estrogen group were increased (P<0.05), but the indicators in Buyang Huan-wu decoction group were higher than estrogen group (P<0.05). Compared with Buyang Huanwu Decoction group, the number of double positive cells and pERK1/2, pCREB1 positive cells in ischemic hippocampal DG area of Buyang Huanwu Decoc-tion+G15 group were decreased (P<0.05). Conclusion Buyang Huanwu Decoction can promote ischemic hippocampal NSCs proliferation in ovariectomized female rats after cerebral ischemia, and to activate ERK/CREB signaling pathway, which may be one of the mechanism of its estrogen-like effect and endogenous neurogenesis.关键词
脑缺血/神经干细胞/ERK/CREB信号通路/去势大鼠/补阳还五汤Key words
cerebral ischemia/neural stem cells/ERK/CREB signaling pathway/OVX rats/Buyang Huanwu Decoction分类
医药卫生引用本文复制引用
黄昕,周胜强,刘胜贤,张必超,薛科辉,刘柏炎..补阳还五汤对去势脑缺血雌性大鼠海马神经干细胞增殖及ERK/CREB信号通路的影响[J].湖南中医药大学学报,2016,36(12):1-6,6.基金项目
国家自然科学基金项目（81273989）；湖南省教育厅一般项目（14C1131）；湖南省科技厅一般项目（S2014F1023）；益阳市科技厅一般项目（YK1541）。 （）
