井冈山大学学报（自然科学版）2016，Vol.37Issue(6)：24-29,6.DOI:10.3969/j.issn.1674-8085.2016.06.007
连云港山楂叶黄酮的超声提取及体外活性研究
STUDY ON EXTRACTION TECHNOLOGY OF FLAVONOIDS FROM HAWTHORN LEAVES WITH ULTRASONIC-ASSISTED METHOD AND BIOACTIVITIES OF THE EXTRACTIN VITRO
摘要
Abstract
Hawthorn leaves as raw material were collected from Huaguo mountain of Lianyungang city and the yield of total flavonoids was used as evaluation indicator. The extraction technology of flavonoids from hawthorn leaves with ultrasonic assisted method was optimized by single and orthogonal experimental design, and the optimal extraction technology was obtained as following: ethanol concentration of 70%, solvent-to-liquid ratio of 20:1 (mL/g), extraction temperature of 70℃, extraction time of 40 min and extraction times of 3. The yield of flavonoids from hawthorn leaves is 2.53%. Moreover, the inhibitory effect of the flavonoids against Staphylococcus aureus, Escherichia coliand Bacillus subtilis was determined by the agar diffusion method, and the antioxidant effect was evaluated by DPPH method. The results showed that the flavonoids extracted from hawthorn leaves had good antibacterial activity and excellent scavenging capacity to DPPH radical.关键词
山楂叶/黄酮类化合物/提取工艺/抑菌活性/抗氧化效应Key words
hawthorn leaves/flavonoids/extraction technology/antibacterial activity/antioxidant effect分类
轻工纺织引用本文复制引用
许瑞波,杨雯雯,万蓓蓓..连云港山楂叶黄酮的超声提取及体外活性研究[J].井冈山大学学报（自然科学版）,2016,37(6):24-29,6.基金项目
江苏省高校自然科学研究基金项目(13KJB150005)；淮海工学院自然科学基金创新基金项目(Z2015015)；江苏省海洋资源开发研究院科技开放基金项目(JSIMR201303) （13KJB150005）
