泸州医学院学报2016，Vol.39Issue(6)：523-526,4.DOI:10.3969/j.issn.1000-2669.2016.06.008
人心房成纤维细胞的体外培养与鉴定
Isolation and culture of human atrial fibroblasts cells in vitro
摘要
Abstract
Objective: To establish a reliable method for isolation and culturing of human atrial fibroblasts. Methods: Hu man atrial fibroblasts were cultured by tissue block method in vitro, an inverted phase contrast microscope was used to observe the morphology, and vimentin was immuno-fluorescence stained to identify the fibroblasts. Results:Human atrial fibroblasts were long spindle shaped or polygonal with transparent cytoplasm, 2~3 nucleuses, and no spontaneous beating; immuno-fluorescence staining showed positive for vimentin. Conclusion:A stable method of fibroblasts isolation and culturing was established, which provides a reliable source of cells for studying myocardial fibrosis related diseases.关键词
成纤维细胞/细胞培养/细胞鉴定Key words
Fibroblast/Cell culture/Cell identification分类
临床医学引用本文复制引用
周伟,李妙龄,范学慧,李涛,王笑宇,于风旭..人心房成纤维细胞的体外培养与鉴定[J].泸州医学院学报,2016,39(6):523-526,4.基金项目
医学电生理学教育部重点实验室开放基金（KeyME-2014-02）；四川省科技厅基金（2011JY0065）资助；泸州市-西南医科大学联合课题 （）
