泸州医学院学报2016，Vol.39Issue(6)：537-541,5.DOI:10.3969/j.issn.1000-2669.2016.06.012
人HP1α/pEGFP-N1真核表达载体的构建及其表达和定位
Construction and expression of human pEGFP-N1-HP1αin eukaryotic cells
摘要
Abstract
Objective: To observe the expression and localization of human HP1α, an eukaryotic expression vector HP1α/EGFP-N1 was constructed and introduced into HeLa cells. Methods:HP1αcDNA were obtained by RT-PCR, and cloned into the eukaryotic expression vector pEGFP-N1 to construct HP1α/EGFP-N1 that was transfected into HeLa cells with Lipofectamine 2000. The expression and location of HP1αin transfected cells was detected by fluorescence microscope. Results: Diagnostic restriction digestion and sequencing analysis verified the identity of the plasmid, HP1α/EGFP-N1. After transfection, HP1α is localized in the nucleus of HeLa cells. Conclusion: Eukaryotic expression plasmid, HP1α/EGFP -N1 is successfully constructed and expressed effectively in HeLa cells.关键词
异染色质相关蛋白/绿色荧光蛋白/基因表达定位/HeLa细胞Key words
HP1a/Enhanced green fluorescent protein(EGFP)/Gene expression/HeLa cells分类
口腔医学引用本文复制引用
吴婷,杨茂,何涛,杨文理..人HP1α/pEGFP-N1真核表达载体的构建及其表达和定位[J].泸州医学院学报,2016,39(6):537-541,5.基金项目
四川省教育厅科研基金资助项目 （）
