中南医学科学杂志2016，Vol.44Issue(6)：637-642,6.DOI:10.15972/j.cnki.43-1509/r.2016.06.008
TSA对发育期大鼠癫痫后脑损伤的保护作用及其机制
The Protection Effects and Mechanisms of TSA on Seizures-Induced Brain Damage in Developing Rat
黄湘壹 1胡擎鹏 2冯伟 2刘剑峰2
作者信息
- 1. 南华大学附属第二医院功能检查科，湖南 衡阳421001
- 2. 南华大学附属第二医院儿科
- 折叠
摘要
Abstract
Objective This study aimed to investigate the protection effects and mechanisms of Histone deacetylase inhibitor ( HDACi) TSA on seizure-induced brain damage of developing rats. Methods 32 male Sprague-Dawley ( SD) rats were randomly divided into 4 groups:Control group,KA group,0.03 mg/kg TSA treated group and 0.1 mg/kg TSA trea-ted group.Intraperitoneal administration of KA induced rat seizures.TSA was injected intraperitoneally 2 hours before KA in-jection.The seizure stage and seizure latency were recorded after KA injection. Hippocampus tissues were sampled at 24 hours after seizures.Brain tissue pathological changes were observed by HE staining;Apoptotic neuron were observed using TUNEL immunohistochemical staining;Activated microglia were detected by CD68 mmunohistochemical staining;IL-1βand TNF-α protein were detected using Western Blot. Results The brain tissue pathological changes,apoptosis of neurons, activated microglia,IL-1β and TNF-αprotein and seizure latency significantly increased after KA treatment ( P<0.05) ,but these effects were attenuated by adding TSA.Compared with 0.03 mg/kg TSA treated group,0.1 mg/kg TSA treated group plays a more important role in protecting against seizure-induced brain damage. Conclusions Histone deacetylase inhibi-tor TSA can reduce brain tissue pathological changes,apoptosis of neurons,activated microglia and inflammation factor.This suggests a protective effect against brain damage associated with seizures in developing rats.关键词
TSA/癫痫/海马/神经元/小胶质细胞Key words
TSA/seizures/hippocampus/neuron/microglia分类
医药卫生引用本文复制引用
黄湘壹,胡擎鹏,冯伟,刘剑峰..TSA对发育期大鼠癫痫后脑损伤的保护作用及其机制[J].中南医学科学杂志,2016,44(6):637-642,6.
