中南医学科学杂志2016，Vol.44Issue(6)：643-647,5.DOI:10.15972/j.cnki.43-1509/r.2016.06.009
miR-21对肺癌干细胞增殖、侵袭和化疗敏感性的影响
Effect of miR-21 on the Capacity of Proliferation,Invasion and Chemo-Sensitivity in Lung Cancer Stem Cells
田艳1
作者信息
- 1. 广州医科大学附属肿瘤医院ICU，广东 广州510095
- 折叠
摘要
Abstract
Objective To investigatet the effect of miR-21 on the capacity of proliferation,invasion and chemo-sen-sitivity in lung cancer stem cells. Methods Lung cancer stem cells were isolated by fluorescence activated cell sorting ( FACS) from human lung adenocarcinoma cell line A549. The miR-21 inhibitors and mimics were transfected into Lung cancer stem cells by liposome.The expression levels of miR-21 in Lung cancer stem cells was measured by reverse transcrip-tion polymerase chain reaction (RT-PCR).MTT method was used to evaluate the capability of cell proliferation and che-moresistance.The invasive capability of cells was evaluated by using transwell chamber model. Results The expression of miR-21 in inhibition group was obviously lower than non-transfection group and negative control group, and in enhanced group it was obviously higher than non-transfection group and negative control group (P<0.05).The proliferation rate at each time point in inhibition group was down-regulated compared with non-transfection group and negative control group,and in enhanced group it was up-regulated compared with non-transfection group and negative control group ( P<0. 05 ) . Transwell chamber assay demonstrated the cell penetrating number was 29.55 ±5.48 in inhibition group at 48 h post-trans-fection,which was obviously less than non-transfection group and negative control group(P<0.05).In enhanced group,the cell penetrating number was 74.47 ±8.63,which was obviously more than non-transfection group and negative control group (P<0.05).The half inhibition concentration (IC50) of Cisplatin (DDP) and Gemcitabine (GEM) in inhibition group was obviously lower than non-transfection group and negative control group.And in enhanced group it was obviously higher than non-transfection group and negative control group ( P<0.05) . Conclusion Down-regulation of miR-21 expression in lung cancer stem cells would inhibit their ability of proliferation and invasion,enhance the sensitivity to chemotherapy.关键词
肺癌干细胞/miR-21/细胞增殖/化疗/耐药性Key words
lung cancer stem cell/miR-21/cell proliferation/chemoresistance分类
医药卫生引用本文复制引用
田艳..miR-21对肺癌干细胞增殖、侵袭和化疗敏感性的影响[J].中南医学科学杂志,2016,44(6):643-647,5.
