山东中医杂志2017，Vol.36Issue(1)：66-70,77,6.
星点设计-响应面法优化热水浸提北沙参多糖的工艺研究
Optimization of Hot Water Extraction Method of Glehnia Littoralis polysaccharides by Central Composite Design and Response Surface Methodology
摘要
Abstract
Objective:To optimize extraction conditions of hot water technology of polysaccharides from Glehnia littoralis by central composite design and response surface methodology. Methods:Polysaccharides of Glehnia littoralis was extracted by hot water extraction method and the content of polysaccharides was deter-mined by phenol sulfuric acid method. Polysaccharides extraction rate was used as the index ,and single fac-tors such as extraction temperature,extraction times,amount of water,times which could affect extract rate was studied. Based on single factor test results ,3 factors and 5 levels central composite design was applied and response surface method was used to optimize the best process. Results:The optimal technology was as follows:in the 25 folds of water,the extraction temperature was at 94.9℃,and extract was performed for 2 times,120 minutes per time. The difference between observed value and predicted value was 4.05% under this condition.Conclusion:The optimized extraction process is stable and feasible ,and the prediction is good.关键词
北沙参/多糖/苯酚-硫酸法/星点设计/响应面法Key words
Glehnia littoralis/polysaccharides/phenol sulfuric acid method/central composite design/response surface methodology分类
医药卫生引用本文复制引用
相美容,王朋展,蒋海强,巩丽丽,吕青涛,容蓉..星点设计-响应面法优化热水浸提北沙参多糖的工艺研究[J].山东中医杂志,2017,36(1):66-70,77,6.基金项目
重大新药创制科技重大专项（编号9509001-001）；山东省高校中医药抗病毒协同创新中心（编号X2014C01-02）；山东省博士后创新项目专项资金资助 （）
