山东农业科学2016，Vol.48Issue(12)：1-5,5.DOI:10.14083/j.issn.1001-4942.2016.12.001
花生 Clp 家族成员的筛选、聚类和盐胁迫响应分析
Screening,Clustering and Response to Salinity Stress of Clp Family Genes in Peanut (Arachis hypogaea L.)
摘要
Abstract
Clp is a kind of ATP -dependent protease widespreading in prokaryote and eukaryote,which plays an important role in resisting biotic and abiotic stresses in plants.To improve the understanding of Clp genes in peanut,28 Clp genes were screened by one transcriptome library constructed with peanut leaves. These 28 Clp genes distributed on 9 chromosomes respectively of A genome of wild peanut.Clustering analysis showed that these 28 Clp genes were assorted to ClpB,C,D,X,P,R,S groups reported previously.To ana-lyze the response to salinity stress of 28 Clp genes,digital gene expression profiles were constructed using a mutant with higher salinity resistance (S2)and its control (S4)before and after treated with 250 mmol /L NaCl.The results showed that 16 Clp genes were responsive to salinity stress in S2 and /or S4.This study pro-vided bases for functional researches and application of Clp genes in peanut.关键词
花生/Clp 基因/RNA 测序/盐胁迫Key words
Peanut (Arachis hypogaea L.)/Clp gene/RNA sequencing/Salinity stress分类
农业科技引用本文复制引用
郑春花,孔祥远,陈冠旭,隋炯明,乔利仙,王晶珊,赵春梅..花生 Clp 家族成员的筛选、聚类和盐胁迫响应分析[J].山东农业科学,2016,48(12):1-5,5.基金项目
国家自然科学基金项目（31571705、31301356、31471542）；山东省科技发展计划项目 （）
