山东农业科学2016，Vol.48Issue(12)：6-15,10.DOI:10.14083/j.issn.1001-4942.2016.12.002
大白菜玻利玛细胞质雄性不育系92－09A 的形态特征及相关基因的克隆与表达模式分析
Morphological Characteristics of Pol CMS Line of Chinese Cabbage (Brassica rapa L.ssp.pekinensis)and Cloning and Expression Pattern Analysis of Related Sterility Genes
摘要
Abstract
Polima cytoplasmic male sterile (Pol CMS)is a very important CMS source in plant breeding. In the previous study,we used Pol CMS line KG -Pol of Chinese cabbage provided by Professor Ke Guilan as female parent and core line 92 -09 as recurrent parent to breed Pol CMS line 92 -09A of Chinese cabbage. Here,the morphological characteristics of Chinese cabbage Pol CMS line 92 -09A,its parental maintainer line 92 -09 and restorer line Zao 28 were investigated,and the Pol CMS gene Braraorf224 and its restorer gene BraraPPR were cloned and analyzed.The results showed that the petals,sepals,stamens and pistils of Chinese cabbage Pol CMS line 92 -09A were all smaller than those of its maintainer line 92 -09,and it could not form mature pollen with viability.While the maintainer line 92 -09 and the F1 hybrid of Pol CMS line 92 -09A ×restorer line Zao 28 both had pollen grains with viability.Bioinformatics analysis indicated that the nucleic acids and amino acids of Chinese cabbage Pol CMS gene Braraorf224 and restorer gene BraraPPR were very close to those of Brassica napus.The results of qRT -PCR showed that the expression level of BraraPPR in restorer line Zao 28 and F1 hybrid were both higher than that in Pol CMS line 92 -09A.The re-search would be helpful to understand the molecular mechanism of Pol CMS of Chinese cabbage in the future.关键词
大白菜/Pol CMS/不育基因/恢复基因Key words
Chinese cabbage (Brassica rapa L.ssp.pekinensis)/Polima cytoplasmic male sterile (Pol CMS)/Male -sterile gene/Restorer gene分类
农业科技引用本文复制引用
王红霞,李景娟,高建伟,李洪雷,郝建梅,丁谦,张一卉,王凤德,邓永林,王均邦,何启伟..大白菜玻利玛细胞质雄性不育系92－09A 的形态特征及相关基因的克隆与表达模式分析[J].山东农业科学,2016,48(12):6-15,10.基金项目
山东省现代农业产业技术体系蔬菜创新团队建设项目（SDAIT －02－022－04）；烟台市科技计划项目（2014NC045）；山东省农业科学院重大成果培育计划项目 （）
