山东农业科学2016，Vol.48Issue(12)：20-24,5.DOI:10.14083/j.issn.1001-4942.2016.12.004
不同外植体和植物激素对大蒜愈伤组织诱导的影响
Effects of Different Explants and Plant Hormones on Callus Induction of Garlic
摘要
Abstract
The effects of different plant hormones on the callus induction of garlic were studied by using the base of clove,shoot tips,root tips and leaves of in vitro plantlet as explants in this study.The results showed that root tips and the base of clove were easier to induce callus than shoot tips and leaves of in vitro plantlet.And the callus induction rate of root tips was the highest in the four kinds of explants.Different plant hormone combinations had different effects on the four kinds of explants.The calli were induced from root tips in all combination of 6 -BA and 2,4 -D.The callus induction rate of the base of clove was 100% in the com-bination of 0.3 mg/L 6 -BA +1.5 mg/L 2,4 -D,or 0.5 mg/L 6 -BA +2.0 mg/L 2,4 -D.The callus induction rate of shoot tips was 85.83% in the best combination of 0.7 mg/L 6 -BA and 1.5 mg/L 2,4 -D. The combination of 0.3 mg/L 6 -BA and 2.0 mg/L 2,4 -D was the best for leaves of in vitro plantlet to in-duce callus,and the rate of callus induction was 71.67%.关键词
大蒜/外植体/植物激素配比/愈伤组织Key words
Garlic/Explant/Plant hormone combination/Callus分类
农业科技引用本文复制引用
孔素萍,杜红霞,杨妍妍,霍雨猛,高莉敏,刘冰江,缪军,吴雄..不同外植体和植物激素对大蒜愈伤组织诱导的影响[J].山东农业科学,2016,48(12):20-24,5.基金项目
山东省农业科学院青年科研基金项目（2015YQN42）；山东省农业良种工程项目“葱姜蒜种质资源创新与利用研究” （）
