山东医药2016，Vol.56Issue(47)：1-3,3.DOI:10.3969/j.issn.1002-266X.2016.47.001
顺铂与XAV939联合应用对人肺癌细胞A549增殖、迁移能力的影响及机制
Effects of cisplatin combined with XAV939 on proliferation,migration and transcription factor expression in WNT pathway of human lung cancer A549 cells
摘要
Abstract
Objective To observe the effects of cisplatin combined with tankyrase small molecule inhibitor XAV939 on the proliferation and migration of human lung cancer A549 cells and to investigate the mechanism.Methods The hu-man lung cancer A549 cells were divided into the blank control group (treated with 1 μmol/L NaCl),cisplatin group (treated with 0.002 g/L),XAV939 group (treated with 1 μmol/L),and cisplatin+XAV939 group (treated with 0.002 g/L cisplatin+1 μmol/L XAV939).The cell proliferation and proliferation inhibitory rates in each group were assessed by MTT assay.The cell migration ability was detected by Scratch test and Transwell chamber assay.Then,the protein expres-sion of tankyrase andβ-catenin was detected by Western blotting,respectively.Results Compared with the control group, the proliferation inhibitory rates of cells was higher,the cell migration distance was shorter,the number of transmembrane cells was less,and the protein expression ofβ-catenin and tankyrase was lower in the cisplatin group,XAV939 group,and cisplatin+XAV939 group (all P<0.05).Compared with the cisplatin and XAV939 group,the proliferation inhibitory rate of the cisplatin +XAV939 group was higher,the cell migration distance was shorter,the number of transmembrane cells was less,and the protein expression ofβ-catenin and tankyrase was lower (all P<0.05 ).Conclusions Cisplatin com-bined with XAV939 may inhibit the proliferation and migration abilities of A549 cells and the combined effect is more pow-erful that of XAV939 or cisplatin alone.This effect may be correlated with the inhibition of β-catenin expression and tankyrase expression.关键词
XAV939/顺铂/肺癌/细胞增殖/细胞迁移/WNT通路/端锚聚合酶/β-连接素Key words
XAV939/cisplatin/lung carcinoma/cell proliferation/cell migration/WNT pathway/tankyrase/β-catenin分类
医药卫生引用本文复制引用
李罛,韩燕燕,郑旭,吕艳,兰福,赵杰..顺铂与XAV939联合应用对人肺癌细胞A549增殖、迁移能力的影响及机制[J].山东医药,2016,56(47):1-3,3.基金项目
天津市卫生局科技基金资助项目（2014KZ130）。 （）
