山东医药2016，Vol.56Issue(47)：25-28,4.DOI:10.3969/j.issn.1002-266X.2016.47.007
ω-3鱼油脂肪乳对肝细胞氧化损伤的修复作用及其机制
Repair function of ω-3 fish oil fat emulsion on oxidative damage in liver cells and its mechanism
摘要
Abstract
Objective To observe the repair function ofω-3 fish oil fat emulsion on oxidative damage in liver cells and to explore its mechanism.Methods Human hepatic cell line HL7702 was cultured in vitro.HL7702 cells were di-vided into the control group,model group and observation group,and each group was divided into 9 holes.The control group was cultured with conventional culture medium,in the model and the observation groups,we added 500 μmol/L hy-drogen peroxide (H2O2)to the culture medium,after 1 hour,the observation group was added with 0.5%ω-3 fish oil fat emulsion,and then we set a blank control group which was only added with the culture medium without cells.After 4 h of culture,the cells in each group were collected.We observed the intracellular lipid droplets by oil red O staining and calcu-lated the cell survival rate by CCK-8,measured reactive oxygen species (ROS)in the cells of each group by DCFH-DA fluorescence probe,detected the alanine aminotransferase (ALT)and aspartate aminotransferase (AST)in the supernatant of each group by colorimetric assay,the triglyceride (TG)and total cholesterol (TC)by COD-PAP single reagent colori-metric method,malondialdehyde (MDA)by thiobarbituric acid and superoxide dismutase (SOD)by xanthine oxidase,the relative expression of peroxisome proliferator-activated receptor-α(PPAR-α)mRNA by real-time fluorescence quantitative PCR,and the relative expression of liver fatty acid-binding protein (L-FABP)protein by Western blotting.Results Un-der microscope,the intracellular lipid droplets were orange.The HL7702 liver cells in the control group arrayed closely, and only a few orange red lipid droplets appeared.In the model group,a large number of orange red lipid droplets were ob-served and accompanied by lipid droplets.In the observation group,the liver cells showed more orange lipid droplets,but they were significantly decreased as compared with those of the model group.The cell survival rates of the control group, the model group and the observation group were 100%,67.25%±4.68%,and 71.72%±2.47%.The survival rate of the observation group was higher than that of the model group (P<0.05),but was lower than that of the control group (P<0.05).Compared with the control group,the intracellular ROS of the model group was increased (P<0.05),the ALT and AST in cell culture supernatants were increased (all P<0.05 ),and the intracellular TG,TC,MDA were increased (all P<0.05),the SOD was decreased (P<0.05),the PPARαmRNA was decreased (P<0.05)and the relative ex-pression of L-FABP protein was decreased (P<0.05).Compared with the model group,the intracellular ROS of the ob-servation group was decreased (P<0.05),the ALT and AST in cell culture supernatants were decreased (all P<0.05), the intracellular TG,TC,MDA were decreased (all P<0.05),the SOD was increased (P<0.05),the PPARαmRNA was increased (P<0.05)and the relative expression of L-FABP protein was increased (P<0.05).Conclusions ω-3 fish oil fat emulsion can repair oxidative damage in human hepatic cell line HL7702,and its mechanism may be related to the up-regulation of the expression of L-FABP/PPARαsignaling pathway and the maintenance of lipid homeostasis in liver cells.关键词
ω-3鱼油脂肪乳/肝细胞/氧化损伤/细胞存活/过氧化物酶体增生物激活受体α/肝型脂肪酸结合蛋白Key words
ω-3 fish oil fat emulsion/hepatic cells/oxidative damage/cell survival/peroxisome proliferator-activa-ted receptor-α/liver fatty acid-binding protein分类
医药卫生引用本文复制引用
李智强,杨大刚,王惠群..ω-3鱼油脂肪乳对肝细胞氧化损伤的修复作用及其机制[J].山东医药,2016,56(47):25-28,4.基金项目
贵州省国际科技合作计划项目（20137023）。 （）
