世界中医药2016，Vol.11Issue(11)：2389-2392,4.DOI:10.3969/j.issn.1673-7202.2016.11.048
藏红花对糖尿病大鼠视网膜神经节细胞凋亡过程中线粒体途径的影响
Effect of Saffron on Mitochondria Pathway during Apoptosis of Retinal Ganglion Cells in Diabetic Rats
摘要
Abstract
Objective:To observe the effect of saffron on mitochondrial function of diabetic rat retinal ganglion cell apoptosis process .Methods:Using random number table method ,72 SD male rats 8 ~10 weeks old with healthy eyes were randomly as-signed to normal control group, diabetic model group, and saffron treatment group (n=24).Normally controlled rats experienced no treatment.Other rats were given streptozocin (SYZ) 25 mg /(kg /D) by intraperitoneal injection for 3 days,once daily, to establish the model of type 2 diabetes and their blood sugar would be more than or equal to 16.7 mmlo/L for a successful model-ing.After the model was established , saffron treatment group was provided with intraperitoneal injection of crocin solution 50 mg /kg, once per day, for 3 consecutive months.And Retinal aldose reductase (AR), neural cell apoptosis and B cell lymphoma /leukemia-2 (Bcl-2), cell pigment and Bcl-2 associated X protein (BCX), cytochrome C (Cyt-C), cespase-3 protein expression were detected .Results:The AR activity in the retina of diabetic rats was significantly higher than that of the normal control group and the treatment group , and the difference was statistically significant ( P<0.05 ); there was small amount of TUNEL positive cells in RCGs layers of the control group , brown staining;B:The RGCs and nuclear layer in diabetic model group had a number of TUNEL staining positive cells; C: there were TUNEL staining positive cells in RGCs and kernel layer in Saffron treatment group, but much less than the number of that of the diabetic model group; Retinal Bcl-2, Bax, Cyt-C and caspase-3 protein ex-pression of diabetic rats were much lower than those of normal control group and treatment group , and the difference is statistically significant (P<0.05).Conclusion:Saffron inhibited AR activity and the activation of the mitochondrial apoptotic pathway , regu-lated expression of apoptosis inhibition of bcl-2 gene and apoptosis promoting gene Bax , Cyt-C and Caspase-3, reduced the apopto-sis of nerve cells in the retina of diabetic rats and showed protective effect on diabetes optic omental injury .关键词
藏红花/糖尿病/视网膜神经节/细胞凋亡/线粒体/功能Key words
Saffron/Diabetes mellitus/Retinal ganglion cells/Apoptosis/Mitochondrial/Function分类
医药卫生引用本文复制引用
党晓洁..藏红花对糖尿病大鼠视网膜神经节细胞凋亡过程中线粒体途径的影响[J].世界中医药,2016,11(11):2389-2392,4.基金项目
国家自然科学基金项目（编号81273902）---藏红花对改善线粒体功能抑制视网膜神经节细胞凋亡的机制研究 （）
