生物信息学2016，Vol.14Issue(4)：203-212,10.DOI:10.3969/j.issn.1672-5565.2016.04.02
伴 HBV 感染性肝癌调控枢纽基因筛选与初步鉴定
Screening and preliminary identification of regulatory hub genes for the HBV-related hepatocellular carcinoma
摘要
Abstract
Primary hepatocellular carcinoma (HCC) caused by chronic hepatitis B virus (HBV) infection involves in a variety of genes, transcripts, proteins and their interactions and regulations with each other .From the perspective of a single gene , the alteration of its expression profile only explains the local occurrence of liver cancer but not in the overall and comprehensive understanding , which is unable to meet needs of the highly complicated disease network studies .Differentially expressed genes screened from the gene chip data of HBV-related HCC were analyzed by the weighted gene co-expression network analysis ( WGCNA ) algorithm to construct the co-expression gene network and identify the HCC-related modules .In this paper , hub genes were visually screened and determined via the gene ontology enrichment analysis with the preliminary identification .Given to the function analysis and the literature surveys , we consistently found that indeed there is a significant association between some genes and the pathogenesis of multiple cancers .WGCNA, approach of system biology , was applied to screen the hub genes for HBV-related HCC and approved to be efficient .SHARPIN, one of the screened hub genes , was found to promote cell migration .The present study has given the novel insight into the understanding of the regulatory mechanism during the progression of liver cancer and the discovery of new diagnosis biomarkers as well as antiviral targets .关键词
乙型肝炎病毒(HBV)/HBV相关性肝癌/GO分析/WGCNA/共表达模块/枢纽基因/SHARPINKey words
HBV-related hepatocellular carcinoma/GO analysis/WGCNA/Co-expression modules/Hub gene/SHARPIN分类
生物科学引用本文复制引用
孙维华,朱祥,尧晨光,孙鸽,寇铮,胡康洪..伴 HBV 感染性肝癌调控枢纽基因筛选与初步鉴定[J].生物信息学,2016,14(4):203-212,10.基金项目
湖北省自然科学基金重点项目（ No．2014CFA075）；武汉市科技局应用基础研究项目（ No．2015060101010033）。 （）
