现代妇产科进展2017，Vol.26Issue(1)：11-14,4.DOI:10.13283/j.cnki.xdfckjz.2017.01.002
IFI16在子痫前期孕妇胎盘组织和血清中的表达及其与子痫前期发病的相关性
Expression of IFI16 in placental tissue and serum of women with preeclampsia and its re-lationship with the pathogenesis of preeclampsia
摘要
Abstract
Objective:To investigate the expression of IFI16 in placental tissue and se-rum of women with preeclampsia and its relationship with the pathogenesis of preeclampsia. Methods:The location of IFI16 in the placenta was detected by immunohistochemistry. IFI16 mRNA and protein levels in the placenta were examined using qRT-PCR and Western blot,re-spectively. Serum IFI16 concentration and ET-1 and sVCAM-1 levels in human umbilical vein endothelial cells ( HUVECs) culture supernate after stimulation with rIFI16 were measured by ELISA. The correlations between serum IFI16 levels with systolic blood pressure ( SBP) ,dias-tolic blood pressure ( DBP) and 24-h proteinuria were analyzed. Results:The positive staining rate of IFI16 in the placenta of women with PE was significantly higher than that in control group ( P<0 . 05 ) . IFI16 mRNA and protein levels were increased in the PE placenta when compared with control placentas ( P<0 . 01 ) . Serum IFI16 level was obviously higher in patients with PE than in control group ( P<0 . 01 ) and positively correlated with SBP ( r=0 . 62 , P<0. 001),the amount of 24h proteinuria (r=0. 723,P<0. 001) and IFI16 expression in corre-sponding placental tissues (r=0. 527,P<0. 05). The concentrations of ET-1 and sVCAM-1 in cell culture supernate after stimulation with rIFI16 were significantly elevated with increasing stimulus levels. Conclusion:IFI16 expression was increased in the placenta tissue and serum of women with PE,which was closely correlated with endothelial dysfunction,suggesting that IFI16 may play a role in PE pathogenesis through detrimental to endothelium.关键词
子痫前期/γ干扰素诱导蛋白16/内皮细胞损伤Key words
Preeclampsia/Interferon-inducible protein 16/Endothelial dysfunction分类
医药卫生引用本文复制引用
张展,王会平,石瑛,贾莉婷,袁恩武,张琳琳,李莹..IFI16在子痫前期孕妇胎盘组织和血清中的表达及其与子痫前期发病的相关性[J].现代妇产科进展,2017,26(1):11-14,4.基金项目
河南省郑州市第一批科技创新项目(No:131PCXTD624) （No:131PCXTD624）
