现代妇产科进展2017，Vol.26Issue(1)：29-32,4.DOI:10.13283/j.cnki.xdfckjz.2017.01.031
联合LC-MS和化学发光免疫法分析不同类型PCOS的雄激素水平
Analysis of androgen levels in different types of PCOS by combined LC-MS and chemi-luminescence immunoassay
摘要
Abstract
Objective:A chemiluminescent immunoassay( CI) in combination with liq-uid chromatography mass spectrometry ( LC-MS ) was used for analysis of serum androgens of women with various clinical types of polycystic ovary syndrome ( PCOS ) , and analyze the bio-chemical characteristics of hyperandrogenism in PCOS women. Methods:One hundred forty-nine PCOS patients with ovulation disorder and polycystic ovaries were recruited. According to their serum Testosterone ( T ) and dehydroepiandrosterone sulfate ( DHEAS ) determined by chemiluminescent assay and their F-G hirsutism score, the subjects were then divided into 2 subgroups:55 hyperandrogenism patients and 94 patients without hyperandrogenism. A control group was composed of 82 patients with tubal infertility. Sex hormone-binding globulin ( SHBG) levels was measured by chemiluminescent immunoassay and then free testosterone index ( FTI) was calculated. LC-MS was used to measure the levels of T, androstenedione ( AD ) and de-hydroepiandrosterone ( DHEA) . Results:Serum T and FTI in hyperandrogenic group were sig-nificantly higher than those in non-hyperandrogenic group and the control ( P<0 . 05 ) . T and FTI in non-hyperandrogenic group were significantly higher than control group ( P<0 . 05 ) . The average levels of DHEAS and AD in both PCOS group were higher than those control group ( P<0. 05). However,the DHEA levels among the 3 groups did not show any significant differ-ences. Conclusions:Based on these results,the levels of T,AD,DHEAS,and FTI in serum were specific parameters for the diagnosis of hyperandrogenism in PCOS patients. Among those pa-rameters,the T level and FTI value were important factors for subtyping of PCOS patients ( hy-perandrogenic or non-hyperandrogenic type) . The LC-MS based method showed agreement with chemiluminescent immunoassay on the diagnosis of hyperandrogenism in PCOS patients.关键词
PCOS/分型/LC-MS/高雄激素血症Key words
PCOS/Subtyping/LC-MS/Hyperandrogenism分类
医药卫生引用本文复制引用
邓小艳,邓艳芹,胡雅君,李金丽,刘莉,肖庆,熊露,吴春林,王敏..联合LC-MS和化学发光免疫法分析不同类型PCOS的雄激素水平[J].现代妇产科进展,2017,26(1):29-32,4.基金项目
武汉市科技局基金(No:2013062301010815) （No:2013062301010815）
武汉市卫计委基金(No:WX14C04) （No:WX14C04）
