现代妇产科进展2017，Vol.26Issue(1)：33-36,4.DOI:10.13283/j.cnki.xdfckjz.2017.01.007
缺氧通过TrkB抑制人滋养细胞系HTR-8/SVneo细胞增殖和侵袭
Hypoxia inhibits cell proliferation and invasion of human trophoblast cell HTR-8/SVneo by inhibiting TrkB
肖艳平 1付久园 1王哲 2葛永梅 1裴美丽3
作者信息
- 1. 承德医学院附属医院产科,承德 067000
- 2. 河北省献县人民医院妇产科,沧州 062250
- 3. 河北省宽城县医院妇产科,承德 067600
- 折叠
摘要
Abstract
Objective:To investigate the effect of hypoxia on the proliferation and inva-sion of human trophoblast cell line HTR-8/SVneo,and explore the possible molecular functional mechanism. Methods:HTR-8/Svneo cells were divided into two groups:cultured in hypoxic conditions and normoxic conditions. CCK8 test and Transwell assay were performed to detect the proliferation and invasion ability of these two groups. Used RT-PCR and Western blot to detect the expressions of HIF-1α mRNA,p-TrkB,TrkB,p-Akt and Akt. Results:The proliferation and invasion of HTR-8/SVneo cell were significantly inhibited after treatment with hypoxia ( P<0. 05). Long duration of hypoxia treatment reduced the expression of HIF-1α mRNA,and the expression of p-TrkB was also significantly inhibited. Moreover,the phosphorylation level of Akt was significantly inhibited ( P<0 . 05 ) . Conclusion:Long duration of hypoxia treatment inhibi-ted the proliferation and invasion of HTR-8/SVneo by inhibiting the activation of TrkB,which led to inhibition of PI3K/Akt signaling pathway.关键词
缺氧/酪氨酸激酶受体B/PI3K/Akt信号通路Key words
Hypoxia/TrkB/PI3 K/Akt signaling pathway分类
医药卫生引用本文复制引用
肖艳平,付久园,王哲,葛永梅,裴美丽..缺氧通过TrkB抑制人滋养细胞系HTR-8/SVneo细胞增殖和侵袭[J].现代妇产科进展,2017,26(1):33-36,4.
