现代妇产科进展2017，Vol.26Issue(1)：37-39,43,4.DOI:10.13283/j.cnki.xdfckjz.2017.01.008
表没食子儿茶素没食子酸酯对人子宫颈癌细胞株HeLa增殖和凋亡的影响
Effect of epigallocatechin-3-gallate on proliferation and apoptosis of HeLa cells
石林 1杨萍 1杜宇1
作者信息
- 1. 四川大学华西第四医院重症监护室,成都 610000
- 折叠
摘要
Abstract
Objective:To investigate the effect and mechanisms of EGCG on the human cervical cancer cells lines HeLa. Methods:The HeLa cell at Logarithmic growth phase were treated with EGCG(0,10,20,40μmol) for 72h. MTT and flow cytometric analysis were used to evaluate the proliferation and the apoptosis of HeLa cell,respectively. The expressions of phos-phorylation p38 mitogen-activated protein kinase ( p-p38 ) , p38 , phosphorylation nuclear tran-scription factorsp-κB ( NF-κB) ,NF-κB,Bax,Bcl-2 and cleaved-Caspase3 in cell were detected by Western blot. The expressions of TNF-α,IL-1β and IL-8 in cell and Cell supernatant were examined by RT-PCR and ELISA, respectively. Results:EGCG can significantly decrease the proliferation of HeLa and reduce the expressions of p-p38,p-NF-κB,Bcl-2,TNF-α,IL-1β and IL-8 in a dose-dependent manner. Further,EGCG treatment also can increase the apoptosis of HeLa and enhance the expression of cleaved-caspase3 and Bax in a dose-dependent manner with no influence on the expression of p38 and NF-κB. Conclusion:EGCG can induces the ap-optosis and growth inhibition of HeLa. The mechanism of which is involved in suppressing p38 /NF-κB mediating inflammation.关键词
表没食子儿茶素没食子酸酯/HeLa/增殖/凋亡/p38/NF-κBKey words
EGCG/HeLa/Proliferation/Apoptosis/p38/NF-κB分类
医药卫生引用本文复制引用
石林,杨萍,杜宇..表没食子儿茶素没食子酸酯对人子宫颈癌细胞株HeLa增殖和凋亡的影响[J].现代妇产科进展,2017,26(1):37-39,43,4.
