中国组织工程研究2016，Vol.20Issue(50)：7469-7474,6.DOI:10.3969/j.issn.2095-4344.2016.50.003
5-氮胞苷诱导骨髓间充质干细胞向心肌细胞分化中的作用
5-Azacytidine induces differentiation of bone marrow mesenchymal stem cells into myocardial cells
摘要
Abstract
BACKGROUND:Bone marrow mesenchymal stem cel s can be differentiated into myocardial cel s induced by 5-azacytidine in vitro, which provides an opportunity for cel transplantation in the treatment of heart disease. OBJECTIVE:To study the induction effects of 5-azacytidine at different concentrations on the myocardial differentiation of bone marrow mesenchymal stem cel s in rats. METHODS:Passage 3 bone marrow mesechymal stem cel s were incubated with DMEM containing 0, 5, 10, 20μmol/L 5-azacytidine for 24 hours, and then the induced medium was replaced by DMEM containing 5%fetal bovine serum for subsequent 28-day culture. Afterwards, expression of cardiac troponin I was detected by immunocytochemical method. RESULTS AND CONCLUSION:Cel death occurred at 24 hours after 5-azacytidine induction, which was more obvious in the 20μmol/L group than the 5 and 10μmol/L groups. The positive expression of cardiac troponin I was significantly lower in the 5μmol/L group than the 10 and 20μmol/L groups (P<0.05), but there was no significant difference between the 10 and 20μmol/L groups (P>0.05). These experimental findings indicate that 5-azacytidine can induce the differentiation of rat bone marrow mesenchymal stem cel s into myocardial cel s in vitro, and its optimal concentration is 10μmol/L.关键词
干细胞/骨髓干细胞/缺血性心脏病/5-氮胞苷/骨髓间充质干细胞/心肌细胞/心肌肌钙蛋白I分类
医药卫生引用本文复制引用
贾秀丽,李昌平..5-氮胞苷诱导骨髓间充质干细胞向心肌细胞分化中的作用[J].中国组织工程研究,2016,20(50):7469-7474,6.基金项目
西南医科大学附属医院基金资助项目(2014-03) （2014-03）
