中国组织工程研究2016，Vol.20Issue(50)：7565-7570,6.DOI:10.3969/j.issn.2095-4344.2016.50.017
酶消化法和组织块法培养心肌干细胞的比较
Enzyme digestion method versus tissue block method in the culture of cardiac stem cells
摘要
Abstract
BACKGROUND:At present, various methods of extracting cardiac stem cel s have been reported in China, but the processes of isolation, culture and purification are not yet standardized. OBJECTIVE:To explore the difference in the culture of rat cardiac stem cel s using enzyme digestion method and tissue block method. METHODS:Cardiac stem cel s from Sprague-Dawley rats, clean grade, 1 day of age, were extracted and cultured in vitro using the enzyme digestion method (control group) or tissue block method (experimental group). RESULTS AND CONCLUSION:Both of the two methods could produce cardiac stem cel s, but the proportion of cultured cel s in the experimental group was significantly higher than that in the control group, indicating the tissue block method is superior to the enzyme digestion method in the culture of cardiac stem cel s.关键词
干细胞/培养/心肌干细胞/细胞培养/免疫荧光/流式细胞术/免疫组化/C-kit+心肌干细胞/河北省自然科学基金分类
医药卫生引用本文复制引用
李沙,李树仁,郝清卿,杨玲玲,张倩辉,马玉龙,张跃华,邓文浩..酶消化法和组织块法培养心肌干细胞的比较[J].中国组织工程研究,2016,20(50):7565-7570,6.基金项目
河北省自然科学基金项目(C2015307019) （C2015307019）
