中国组织工程研究2016，Vol.20Issue(51)：7634-7641,8.DOI:10.3969/j.issn.2095-4344.2016.51.005
保留残端与不保留残端重建前交叉韧带腱骨愈合情况比较
Tendon-bone healing after acute anterior cruciate ligament reconstruction with versus without remnant preservation
摘要
Abstract
BACKGROUND:Reconstruction with remnant preservation can enhance tendon-bone healing. However, the study limits on the histological level, and there is a lack of research based on the modular biological level. <br> OBJECTIVE:To investigate whether anterior cruciate ligament reconstruction with remnant preservation can enhance tendon-bone healing. <br> METHODS:Seventy-two New Zealand rabbits were randomly al ocated to three groups (n=24 per group), fol owed by cruciate ligament reconstruction without remnant (group A), with remnant preservation (femoral tensioning and augmented suture) (group B) and with remnant preservation (graft passing remnant anterior cruciate ligament sheath) (group C), respectively. <br> RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that the tendon-bone healing in the groups B and C surpassed that in the group A, and group B was better than group C. Real-time PCR revealed that the expression level of osteoprotegrin mRNA and the osteoprotegrin/receptor activator of nuclear factor-κB ligand (RANKL) ratio were greater in the groups B and C than in the group A, and highest in the group C, while the expression levels of RANKL mRNA in the groups B and C were lower than that in the group A. In conclusion, these two kinds of anterior cruciate ligament reconstruction methods with remnant preservation can enhance tendon-bone healing, which have obtained most obvious achievements in the anterior cruciate ligament reconstruction in the graft passing anterior cruciate ligament remnant sheath that may be related to the up-regulation of osteoprotegrin mRNA and down-regulation of RANKL mRNA.关键词
组织构建/组织工程/重建前交叉韧带/保留残端/腱骨愈合/骨保护素/细胞核因子κB 受体活化因子配体分类
医药卫生引用本文复制引用
张磊,岳娜,张太良,谢崇新,白靖平..保留残端与不保留残端重建前交叉韧带腱骨愈合情况比较[J].中国组织工程研究,2016,20(51):7634-7641,8.基金项目
新疆生产建设兵团自然科学基金资助项目(2015AD004)Funding:the Natural Science Foundation of the Xinjiang Production and Construction Corps, No.2015AD004 （2015AD004）
