中国组织工程研究2016，Vol.20Issue(52)：7781-7787,7.DOI:10.3969/j.issn.2095-4344.2016.52.004
钛离子对T淋巴细胞体外增殖及活化的影响
Effects of titanium ions on the proliferation and activation of T lymphocytes in vitro
摘要
Abstract
BACKGROUND:Titanium ions have been proved to stimulate the secretion of bone remodeling-related factors from T lymphocytes;however, the effects of titanium ions on the early activation, intermediate activation, and cel cycle of T lymphocytes remain unclear. OBJECTIVE:To investigate the effects of titanium ions on the proliferation and activation of T lymphocytes in vitro. METHODS:Cel proliferation and cycle test:Jurkat E6-1 T lymphocytes in logarithmic phase were col ected and cultured in the medium containing 0 (control), 25 (low concentration), 50 (middle concentration), and 100μmol/L (high concentration) titanium ions for 24 hours to detect the cel relative proliferation rate and cel cycle. Cel activation trial:Jurkat E6-1 T lymphocytes were divided into two groups that were subdivided into four groups containing 0, 25, 50, and 100μmol/L titanium ions, respectively with or without phytohemagglutinin (PHA) pre-stimulation. The expressions of CD69 and CD25 were measured after cultured for 24 hours. RESULTS AND CONCLUSION:Titanium ions enhanced T lymphocytes proliferation in a concentration-dependent manner (P<0.05). Compared with the control group, the percentages of G0/G1 phase decreased and the proportions of cel s in S and G2/M phase increased significantly in the low, middle and high concentration groups (P<0.05). The proportion of G0/G1-phase cel s in the high concentration group was less and the proportion of G2/M phase cel s was higher than those in the middle and low concentration groups (P<0.05). With PHA pre-stimulation, the expression of CD69 in the high concentration group was higher than that in the middle and low concentration groups (P<0.05);whereas the difference of CD25 expression was not significant among four subgroups. Titanium ions promoted the expression of CD69 in a concentration-dependent manner (P<0.05), but there was no CD25 expression in each subgroup without PHA pre-stimulation. To conclude, titanium ions can significantly promote T lymphocyte proliferation and early activation in vitro, and moreover, induce S and G2/M phase arrest in T lymphocytes.关键词
生物材料/口腔生物材料/种植体无菌性松动/T淋巴细胞/骨免疫/钛离子/骨吸收/增殖/细胞周期/CD69/CD25/破骨细胞/种植体周围炎/国家自然科学基金分类
医药卫生引用本文复制引用
陈富军,陈东晖,杨倩,李昌,唐礼..钛离子对T淋巴细胞体外增殖及活化的影响[J].中国组织工程研究,2016,20(52):7781-7787,7.基金项目
国家自然科学基金项目(81160136，81460108)@@@@the National Natural Science Foundation of China, No.81160136,81460108 （81160136，81460108）
