中国组织工程研究2016，Vol.20Issue(52)：7851-7857,7.DOI:10.3969/j.issn.2095-4344.2016.52.014
3D打印羊椎骨粉/聚乙烯醇支架、纳米级羟基磷灰石/聚乙烯醇支架、羊椎骨粉/聚乙烯醇无孔骨板的性能比较
Performance comparison of 3D printing sheep vertebral bone meal/polyvinyl alcohol scaffold, nano-hydroxyapatite/polyvinyl alcohol scaffold and sheep vertebral bone meal/polyvinyl alcohol nonporous bone plate
摘要
Abstract
BACKGROUND:With the promotion of 3D printing technology, 3D printing scaffolds for bone tissue engineering have become the new ideas for jaw bone repair. OBJECTIVE:To compare the physical and biological properties of sheep vertebral bone meal/polyvinyl alcohol (PVA) scaffold, nano-hydroxyapatite (nHA)/PVA scaffold, and sheep vertebral bone meal/PVA nonporous bone plate. METHODS:3D printing technology was used to print sheep vertebral bone meal/PVA scaffold, nHA/PVA scaffold, and sheep vertebral bone meal/PVA nonporous bone plate. Porosity, morphology, water absorption rate and mechanical properties of different scaffolds were detected. Three kinds of scaffolds were al used to culture bone marrow mesenchymal stem cel s, and cel proliferation ability was detected using cel counting kit-8 at 1, 4, 7 days of culture. RESULTS AND CONCLUSION:Under scanning electron microscope, the sheep vertebral bone meal/PVA scaffold and nHA/PVA scaffold exhibited regular and interconnected pores with good continuity and clear network structure;the sheep vertebral bone meal/PVA nonporous bone plate had no obvious pores;however, it had dense and evenly distributed micropores with different sizes on its surface. The porosity of nHA/PVA scaffold was lower than that of the sheep vertebral bone meal/PVA scaffold (P<0.05). The water absorption rate was highest for the nHA/PVA scaffold fol owed by the sheep vertebral bone meal/PVA scaffold and the sheep vertebral bone meal/PVA nonporous bone plate (P<0.05). In contrast, the scaffold toughness was highest for the sheep vertebral bone meal/PVA nonporous bone plate, fol owed by the sheep vertebral bone meal/PVA scaffold and nHA/PVA scaffold. In addition, the cel proliferation activity of cel s cultured on the sheep vertebral bone meal/PVA scaffold was significantly higher than that cultured on the other two kinds of scaffolds. Taken together, the 3D printing sheep vertebral bone/PVA scaffold has good physical and chemical performance.关键词
生物材料/骨生物材料/组织工程骨/3D打印/羟基磷灰石/羊椎骨粉/聚乙烯醇分类
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周琦琪,韩祥祯,宋艳艳,吕明凡,胡杨,何惠宇..3D打印羊椎骨粉/聚乙烯醇支架、纳米级羟基磷灰石/聚乙烯醇支架、羊椎骨粉/聚乙烯醇无孔骨板的性能比较[J].中国组织工程研究,2016,20(52):7851-7857,7.基金项目
新疆维吾尔自治区科技厅科技支疆项目(201291173)@@@@the Scientific Support Program for Xinjiang Uygur Autonomous Region, China, No.201291173 （201291173）
