摘要
Abstract
Objective To study the effect of abnormal blood flow stress or pressure solely on expressions of Toll-like receptor 4 (TLR4)/NF-κB signaling pathway and downstream inflammatory factors in human umbilical vein endothelial cells (HUVECs) ,name-ly lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) ,tumor necrosis factor-α (TNF-α) ,intercellular cell adhesion molecule-1 (ICAM-1) ,vascular cell adhesion molecule-1 (VCAM-1) ,as well as its possible mechanisms leading to atherosclerosis . Methods The HUVECs were divided into stress group ,pressure group and normal group according to the different stresses .The 4th , 5th generation of HUVECs were inoculated in the cell synthetic stress stimulation test system (model bio-ccs13) at 1 × 105 cells/ml . The cells were collected respectively 24 hours after the stress of each group .The gene expressions of TLR4 ,myeloid differentiation factor 88 (MyD88) ,tumor necrosis factor receptor-associated factor-6 (TRAF-6) ,LOX-1 ,NF-κB ,TNF-α ,VCAM-1 and ICAM-1 were detected by qPCR ,and the protein expressions of TLR4 ,NF-κB ,LOX-1 ,TNF-α ,ICAM-1 and VCAM-1 by Western blot .Re-sults Compared with the normal group ,mRNA expressions of TLR4 ,MyD88 ,TRAF-6 ,LOX-1 ,NF-κB ,TNF-α ,VCAM-1 and I-CAM-1 increased significantly (P< 0 .01) in the stress group and pressure group ;protein expressions of TLR4 ,NF-κB ,LOX-1 , TNF-α ,VCAM-1 and ICAM-1 also increased significantly (P< 0 .01) .The difference was statistically significant .Conclusions The mechanism of abnormal blood flow stress or pressure leading to atherosclerosis might be associated with the activation of TLR 4/NF-κB signal transduction pathway and the enhancement of its downstream inflammatory factors ,such as the expressions of LOX-1 ,TNF-α , VCAM-1 and ICAM-1 .关键词
Toll 样受体 4/壁面压力/张应力/壁面切应力/动脉粥样硬化Key words
Toll like receptor 4/wall pressure/circumferential strain/wall shear stress/Atherosclerosis分类
医药卫生
