医药导报2017，Vol.36Issue(1)：65-69,5.DOI:10.3870/j.issn.1004-0781.2017.01.016
一测多评法测定肾炎四味颗粒中4种黄酮类成分的含量
Determination of Four Flavonoids in Shenyan Siwei Granules by Quantitative Analysis of Multi-components with Single Marker
周伟 1曹全胜1
作者信息
- 1. 湖北省食品药品监督检验研究院,武汉 430064
- 折叠
摘要
Abstract
Objective To establish an HPLC method to measure four flavonoids ( baicalin, wogonoside,baicalein and wogonin) in shenyan siwei granules by quantitative analysis of multi-components with single marker ( QAMS ) . Methods Agilent ZORBAX SB-C18 column(4. 6 mm × 250 mm,5 μm) was used. The mobile phase was composed with methanol and 0. 4% phosphoric acid solutionat at 1. 0 mL·min-1 flow rate with gradient elution. The detection wavelength was 278 nm. Baicalin was used as the internal reference substance. The relative correction factors ( RCF) between the baicalin and the other three flavonoids were established to detect the quantitation of baicalin and calculate the quantitation of the other three constituents. The external standard method was used for quantitating the four constituents, and the method was evaluated by comparing to the quantitative results between external standard method and QAMS method. Results The results of QAMS method had no significant difference with those of external standard method. Conclusion It is feasible and accurate to control the quality of shenyan siwei granules with QAMS.关键词
肾炎四味颗粒/黄芩苷/汉黄芩苷/黄芩素/汉黄芩素/一测多评/校正因子/色谱法,高效液相Key words
Shenyan siwei granules/Baicalin/Wogonoside/Baicalein/Wogonin/Quantitative analysis of multi-components with single marker/Relative correction factor/Chromatography/high performance liquid分类
医药卫生引用本文复制引用
周伟,曹全胜..一测多评法测定肾炎四味颗粒中4种黄酮类成分的含量[J].医药导报,2017,36(1):65-69,5.
