中国感染控制杂志2016，Vol.15Issue(12)：913-916,933,5.DOI:10.3969/j.issn.1671-9638.2016.12.004
吴江地区多重耐药鲍曼不动杆菌碳青霉烯酶基因携带情况及同源性
Carriage and homology of carbapenemase genes of multidrug-resistant Acinetobacter baumannii in Wuj iang
摘要
Abstract
Objective To investigate the carriage and homology of carbapenemase genes of multidrug-resistant Acinetobacterbaumannii (MDRAB)in Wujiang area.Methods A total of 44 non-duplicated MDRAB isolated from patients in 3 general hospitals in Wujiang area from January 2010 to December 2013 were collected. Minimum inhibitory concentrations (MICs)were detected,carbapenemase genes OXA-51,OXA-23,OXA-24,OXA-58, IMP,TEM,SHV,and GES were amplified with polymerase chain reaction(PCR),homology of strains was detec-ted with pulsed-field gel electrophoresis (PFGE).Results 44 MDRAB strains were mainly collected from sputum (93.18% ),mainly distributed in intensive care unit (ICU),department of respiratory diseases,and department of neurosurgery,accounting for 45.45% ,27.27% ,and 13.64% respectively;MDRAB were both sensitive to minocy-cline and polymyxin B,resistance rates to piperacillin,ampicillin/sulbactam,ceftazidime,gentamicin,amikacin, and ciprofloxacin were all 100.00% ,resistance rates to imipenem and meropenem were both 97.73% . 44 MDRAB strains were all detected OXA-51,OXA-23 and TEM genes,12 strains were positive for GES gene,1 strain was positive for OXA-58 and SHV respectively,OXA-24 and IMP genes were not found ;MDRAB were divided into 7 types of G-A,which included 19,3,9,3,1,4,and 5 strains respectively,type A was mainly from two large gen-eral hospitals in Wujiang area (Wujiang First People’s Hospital and Shengze Hospital),type B,D and E strains were not detected in Wujiang First People’s Hospital,type E strain was only 1 isolate and was from Yongding Hos-pital,the other types were sporadically distributed.Conclusion Multidrug resistance of clinically isolated Acineto-bacterbaumannii is serious in Wujiang area,OXA-23 and TEM genes are major causes of multidrug resistance in Acinetobacterbaumannii,the main types are A and C,which present clonal spread.关键词
鲍曼不动杆菌/碳青霉烯酶基因/多重耐药/脉冲场凝胶电泳/PFGEKey words
Acinetobacterbaumannii/carbapenemase gene/multidrug resistance/pulsed-field gel electrophoresis/PFGE分类
医药卫生引用本文复制引用
朱善军,倪晓艳,吴巧珍,沈国荣,陆静芬,朱同华,沈昊,马春芳..吴江地区多重耐药鲍曼不动杆菌碳青霉烯酶基因携带情况及同源性[J].中国感染控制杂志,2016,15(12):913-916,933,5.基金项目
吴江第一人民医院基金资助项目 （）
