中国感染控制杂志2016，Vol.15Issue(12)：934-938,5.DOI:10.3969/j.issn.1671-9638.2016.12.009
马尔尼菲青霉菌鉴定要点及该菌酵母相体外药敏分析
Identification and in vitro antifungal susceptibility of Penicillium marneffei in yeast phase
摘要
Abstract
[Abstract ] Objective To understand the identification and in vitro antifungal susceptibility of Penicillium marneffei(PM)in yeast phase,and guide clinic antifungal application.Methods Strains isolated from blood and bone marrow of 23 patients infected with PM in a hospital between 2009 and 2016 were collected,colony morpholo-gy of PM in yeast phase was observed,susceptibility to itraconazole,voriconazole,amphotericin B,and fluconazole were detected with E-test method.Results Colony morphology of PM were as follows:direct microscopic examina-tion of Wright’s staining of tissue specimens found visible oval or round spore with apparent septum,and mainly lo-cated in macrophage;Gram staining of blood culture specimens found that strains were with bulbous and slightly curved ends,occasionally branched and with septum. PM was dimorphic fungi,presented mycelium at 28° C,pro-duced red pigment and diffused into medium;PM presented yeast form at 35° C,there were typical colony morpholo-gy. Minimum inhibitory concentrations (MICs)of itraconazole,voriconazole,amphotericin B,and fluconazole to PM in yeast phase were 0.002-0.016,0.012-0.125,0.002-0.500,and 0.500-16.000μg/mL respectively. Conclusion Typical colony morphology and fungal spore of PM in bone marrow and peripheral blood are important features for identification. PM is most susceptible to itraconazole,followed by voriconazole and amphotericin B, while fluconazole is less susceptible.关键词
马尔尼菲青霉病/马尔尼菲青霉菌/双相型/抗药性,微生物/耐药性/真菌Key words
Penicilliosis marneffei/Penicilliummarneffei/dimorphic fungus/drug resistance,microbial/fungus分类
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谢良伊,王娟,张景,程振波,李浩,梁剑,郑淑娟,曾玲,邓中华..马尔尼菲青霉菌鉴定要点及该菌酵母相体外药敏分析[J].中国感染控制杂志,2016,15(12):934-938,5.基金项目
湖南省人民医院仁术基金资助项目 （）
