中国现代医学杂志2016，Vol.26Issue(24)：6-10,5.DOI:10.3969/j.issn.1005-8982.2016.24.002
基于环介导等温扩增技术建立的假丝酵母菌快速检测方法
Establishment of loop-mediated isothermal amplification forrapid detection of candida spp
摘要
Abstract
Objective To establish a rapid detection of candida spp on the basis of loop-mediated isothermal method. Methods According to the gene bank, 18 S rDNA gene sequence was provided. Primer Explorer V4 online design software design for the Candida genus-specific primers was used, and LAMP expansion method and the reaction system was set up. Four key parameters for the system and three performance were optimized and discussed, and then verified by clinical samples. Results Six kinds of candida were all positive, and the optimum temperature was 63℃ - 65℃, the activity of Bst was 4 u, concentration of Mg2+was 4 mM, time was 1 h. All 7 kinds of clinical reference strains and Human whole blood DNA samples amplification results were negative. The detection limit was 107-102 CFU/ml. A total of 52 cases of clinical samples proved that the method sensitivity was 100%, and the specificity was 95%. Conclusions The method of the genus candida established by LAMP technology is significantly practical, simple, rapid, specific and sensitive.关键词
假丝酵母菌/环介导等温扩增/检测方法Key words
candida/loop-mediated isothermal amplification/detection methods分类
医药卫生引用本文复制引用
林江,温先勇,林雁,邓正华,黄远帅..基于环介导等温扩增技术建立的假丝酵母菌快速检测方法[J].中国现代医学杂志,2016,26(24):6-10,5.基金项目
西南医科大学青年科技基金 （）
