中国现代医学杂志2016，Vol.26Issue(24)：24-27,4.DOI:10.3969/j.issn.1005-8982.2016.24.005
结核分枝杆菌临床分离株利福平和异烟肼耐药性检测方法比较
Comparison of two methods detect of Mycobacterium tuberculosis clinical isolates resistance to rifampin and isoniazid
摘要
Abstract
Objective To evaluate the use of gene chip, Lowenstein-Jensen (L-J) culture method and proportion method in detecting Mycobacterium tuberculosis (MTB) resistant of rifampinc (RFP) and isoniazid (INH). Methods A total of 200 MTB clinical isolates were chosed, 6 sites of resistant gene rpoB and 13 kinds of mutant of RFP were detected, and 1 site of resistant gene katG, 2 kinds of mutant and inhA gene promoter-15, 1 kind of C→T mutant of INH were detected. Meanwhile drug susceptibility of the 200 MTB clinical isolates with rapid culture and proportion method were detected. Then the sensitivity, specificity and accuracy were compared. Results A number of 152 sensitive isolates and 48 resistant isolates were detected by gene chip, and 161 sensitive isolates and 39 resistant were isolated by proportion method. If the result of proportion method was set as criteria, the sensitivity, specificity and accuracy of gene chip test RFP drug were 85%, 98.9% and 97.5%, for INH they were 82.8%, 92.4%, 91%. Conclusions Gene chip method can be used to preliminarily screen the drug-resisitant strains of Mycobacterium tuberculosis. Joint detection with proportion method can improve the diagnostic rate.关键词
分枝杆菌,结核/药物敏感性/基因芯片/比例法/异烟肼/利福平Key words
mycobacterium tuberculosis/drug susceptibility/gene chip/proportion method/isoniazid/rifampin分类
医药卫生引用本文复制引用
李同霞,江国峰,郝文嘉,曹佳伟,苏海涛,肖谦,王丙瑞..结核分枝杆菌临床分离株利福平和异烟肼耐药性检测方法比较[J].中国现代医学杂志,2016,26(24):24-27,4.基金项目
青岛市科技局公共领域科技支撑计划课题 （）
