中国药理学通报2016，Vol.32Issue(12)：1648-1656,9.DOI:10.3969/j.issn.1001-1978.2016.12.005
银杏二萜内酯对缺血／再灌注大鼠脑组织中神经递质的影响
Effects of diterpene ginkgolides meglumine injection on neurotransmitters in cerebral ischemia-reperfusion injury rats
摘要
Abstract
Aim To investigate the effects of diterpene ginkgolides meglumine injection (DGMI ) on amino acids and monoamine neurotransmitters in rats with cerebral ischemia/reperfusion injury.Methods In-traluminal suture was applied to establish middle cere-bral artery occlusion (MCAO/R)model with ischemia for 1.5 h and reperfusion for 24 h.After the adminis-tration of DGMI (i.v.),the levels of amino acid and monoamine neurotransmitters in brain tissue were de-tected through HPLC-ECD.Results DGMI down-reg-ulated the concentrations of aspartic acid, glutamic acid,glycine and γ-aminobutyric acid which were in-creased in MCAO/R group.DGMI also reduced the levels of norepinephrine epinephrine,glyoxylic acid, serotonin and 5-HIAA in cortex and hippocampus,and increased adrenaline content compared to the model group.Conclusion DGMI exhibits a protective role in rats with cerebral ischemia /reperfusion injury through regulating amino acids and monoamine neuro-transmitters.关键词
银杏二萜内酯葡胺注射液/脑缺血/再灌注/氨基酸类递质/单胺类递质/HPLC-ECD/代谢组学Key words
DGMI/cerebral ischemia-reperfusion/a-mino acid neurotransmitters/monoamine neurotrans-mitters/HPLC-ECD/metabonomics引用本文复制引用
张雯,杜冠华,宋俊科,何国荣,张雪,周启蒙,王振中,萧伟,肖智勇,周文霞..银杏二萜内酯对缺血／再灌注大鼠脑组织中神经递质的影响[J].中国药理学通报,2016,32(12):1648-1656,9.基金项目
国家科技部“重大新药创制”科技重大专项资助项目（No 2013ZX09508104，2013ZX09402203）；抗毒药物与毒理学国家重点实验室开放课题（No TMC201510）；国家自然科学青年基金项目（No 81603100）；北京协和医学院研究生创新基金项目 （）
