中国药理学通报2016，Vol.32Issue(12)：1670-1676,7.DOI:10.3969/j.issn.1001-1978.2016.12.008
大蒜活性成分DATS抑制血小板激活因子介导的黑色素瘤转移的实验研究
Experimental research of inhibitory effects of garlic active ingredients DATS on PAF-mediated melanoma metastasis
摘要
Abstract
Aim To discuss the impact of important ac-tive ingredient of garlic diallyl sulfide———DATS on platelet activating factor (PAF ) mediated melanoma metastasis and its mechanisms.Methods ①MTT was used to test the effect of different concentrations of DATS on B16F10 and A375 melanoma cell growth number;②Scratch test and transwell were employed to test the effect of different concentrations of DATS on B16F10 and A375 melanoma cell migration;③ West-ern blot was used to test the effect of DATS on expres-sion of MMP-2,ERK,p38 induced by PFA;④Intrave-nous injection of tumor metastasis model was used to check the inhibition of DATS in PAF-mediated melano-ma metastasis.Results B16F10 cells relative growth rate fell to 73.21% and 48.78%,respectively,when DATS concentration reached 50 and 100 μmol·L-1 . DATS inhibited the levels of PAF-induced migration of melanoma cells B16F10 and vertical migration signifi-cantly,and inhibited B16F10 cells migration induced by PAF through inhibiting the expression of MMP-2, paxillin protein,FAK and other proteins.Conclusion DATS can significantly inhibit PAF-induced tumor metastasis, which is related to the inactivation of MAPKs.关键词
大蒜素/黑色素瘤/肿瘤转移/DATS/PAF/MAPKKey words
allicin/melanoma/tumor metastasis/DATS/PAF/MAPK引用本文复制引用
沈颖,刘玉萍,王旭,沈培亮,祝娉婷,陈文星,王爱云,陆茵..大蒜活性成分DATS抑制血小板激活因子介导的黑色素瘤转移的实验研究[J].中国药理学通报,2016,32(12):1670-1676,7.基金项目
国家自然科学基金资助项目（No 81403260，81573859）；中国博士后科学基金（No 2014M551639）；江苏省自然科学基金资助项目（No 1401138C）；2013年江苏高校优秀科技创新团队计划［苏教科（2013）10号文］；江苏高校品牌专业建设工程资助项目（No PPZY2015A070）；江苏高校中药学优势学科建设工程资助项目（PAPD ）［苏政办发（2014）37号文］ （）
