中国药理学通报2016，Vol.32Issue(12)：1683-1687,5.DOI:10.3969/j.issn.1001-1978.2016.12.010
安息香对永久性大脑中动脉栓塞大鼠模型的保护作用及其机制研究
Study of protective effects and mechanism of benzoinum against permanent focal cerebral ischemia injury in rats
摘要
Abstract
Aim To discuss protective effects and the mechanism of benzoinum against permanent focal cere-bral ischemia (pMCAO)injury in rats.Methods sixty-five adult male Sprague-Dawley rats, weighing 230 ~270g were randomly divided into five groups:sham,pMCAO,pMCAO +vehicle,pMCAO +nimo-dipine,pMCAO +benzoinum groups.The rats were subjected to permanent focal cerebral ischemia.Neuro-logical deficit testing was performed with Longa ’s Scale,and ischemic brain tissue water content as well as brain edema rate was calculated.Cerebral infarction rate was tested by TTC-staining. Serum content of VEGF and TNF-αwas detected by ELISA.Results Compared with pMCAO +vehicle group,benzoinum had a tendency to improve the neurological deficit,and could significantly decrease the water content of ische-mic brain tissue,brain edema rate and cerebral infarc-tion rate (P<0.01 ).Benzoinum also could increase serum levels of VEGF (P<0.01)and decrease serum levels of TNF-α(P<0.05 )in pMCAO model rats. Conclusions Benzoinum can reduce the ischemic brain tissue water content,brain edema rate and cere-bral infarction rate,playing a role in cerebral protec-tion,which may be the biological basis for inducing re-suscitation.Its mechanism may be correlated with the up-regulation of VEGF and down-regulation of TNF-α.关键词
安息香/永久性局灶性脑缺血/神经功能评分/脑含水量/脑梗死率/血管内皮细胞生长因子/肿瘤坏死因子-αKey words
benzoinum/permanent focal cerebral is-chemia/neurological score/ischemic brain tissue wa-ter content/cerebral infarction rate/VEGF/TNF-α引用本文复制引用
文静,王建,陈念,罗世兰,谢倩,马骁..安息香对永久性大脑中动脉栓塞大鼠模型的保护作用及其机制研究[J].中国药理学通报,2016,32(12):1683-1687,5.基金项目
国家自然科学基金面上资助项目 （）
