中国医学科学院学报2016，Vol.38Issue(6)：660-665,6.DOI:10.3881/j.issn.1000-503X.2016.06.007
四氧嘧啶诱导水蚤糖尿病模型的建立
Establishment of An Alloxan-induced Diabetes Model in Daphnia Pulex
摘要
Abstract
Objective To establish a Daphnia model of alloxan-induced diabetes. Methods Daphnia were exposed to three different concentrations of alloxan (3, 5, and 10 mmol/L) for 30 minutes. Blood glucose and survival rate were recorded for 72 hours after alloxan insult. Sequence analysis and phylogenetic inference for glucose transporters ( GLUT ) were clustered with the maximum-likelihood method. Using reverse transcription and quantitative polymerase chain reaction techniques, we investigated the transcriptional changes of GLUT at 12 hours after alloxan (5 mmol/L) exposure. Results Compared with control, 3 mmol/L, and 5 mmol/L as well as 10 mmol/L alloxan initially induced transient blood glucose decline by 15% for 2 hours and 12 hours respec-tively. In Daphnia with 5 and 10 mmol/L alloxan, their blood glucose was persistently raised by about 150%since after 24-hour insult. Survival rate of Daphnia exposure to alloxan with concentrations of 3, 5, and 10 mmol/L were 90%, 75%, and 25% respectively. We predicted seven GLUT genes in the Daphnia genome and success-fully amplified them using real-time polymerase chain reaction. Two of seven GLUT transcripts were down-regula- <br> ted in Daphnia with 5 mmol/L alloxan-induced diabetes. Conclusion Alloxan-induced diabetes model was suc-cessfully established in the Daphnia pulex, suggesting diabetes-relevant experiments can be conducted using Daphnia.关键词
水蚤/糖尿病/葡萄糖转运蛋白/四氧嘧啶Key words
Daphnia/diabetes/glucose transporter/alloxan分类
医药卫生引用本文复制引用
胡安民,朱涛,江叶,董丽,高鸿,杜桂芝..四氧嘧啶诱导水蚤糖尿病模型的建立[J].中国医学科学院学报,2016,38(6):660-665,6.基金项目
国家自然科学基金(81202607) Supported by the National Natural Sciences Foundation of China (81202607) （81202607）
