中国医学科学院学报2016，Vol.38Issue(6)：710-714,5.DOI:10.3881/j.issn.1000-503X.2016.06.014
荧光素二乙酸酯荧光微孔板法在细胞活力测定中的应用
Fluorescein Diacetate Microplate Assay in Cell Viability Detection
摘要
Abstract
Objective To investigate the application of the fluorescein diacetate ( FDA) microplate as-say in cell viability detection. Methods Cells were seeded in a 96-well culture plate until detection. After incu-bated with FDA, the plate was detected by fluorescence microplate analyzer. The effects of FDA incubation dura-tion, concentration, and other factors on the assay’ s accuracy and stability were assessed. We also compared the results of FDA with methyl thiazolyl ( MTT) in terms of cell numbers and H2 O2 injury. Results Within 0-30 minutes, the fluorescence-cell number coefficient of FDA assay increased with duration and reached 0. 99 in 27-30 minutes. The optimum concentration of final FDA in this study was 10-30 μg/ml. On cell viability detec-tion, the result of FDA method was equivalent to MTT method. As to H2 O2 injury assay, the sensitivity of FDA method was superior to MTT on the higher concentration H2 O2 treatment due to a relative shorter duration for de-tection. Conclusion As a stable and reliable method, FDA is feasible for cell variability detection under varied conditions.关键词
荧光素二乙酸酯/荧光/微孔板检测/细胞活力/四甲基偶氮唑盐/细胞毒Key words
fluorescein diacetate/fluorescence/microplate assay/cell viability/methyl thiazolyl/cytotoxicity分类
生物科学引用本文复制引用
陈熙,杨秀颖,方莲花,杜冠华..荧光素二乙酸酯荧光微孔板法在细胞活力测定中的应用[J].中国医学科学院学报,2016,38(6):710-714,5.基金项目
国家自然科学基金(81470159)和中国医学科学院医学与健康科技创新工程(2016-I2M-3-007) Supported by National Natural Sciences Foun-dation of China (81470159), and CAMS Initiative for Innovative Medicine (CAMS-I2M)(2016-I2M-3-007) （81470159）
