中国医学装备2016，Vol.13Issue(12)：150-153,154,5.DOI:10.3969/J.ISSN.1672-8270.2016.12.044
孕激素和脂联素分子受体11对A549肺癌细胞侵袭转移的影响研究
Study of the effect of PAQR11 on invasion and metastasis in A549 lung cancer cells
摘要
Abstract
Objective:To investigate the effect of PAQR11 on invasion and metastasis in A549 lung cancer cells, and explore the possible molecular mechanism.Methods: Two oligonucleotides for interfering PAQR11 were designed, and were cloned into pLKO.1 lentiviral plasmid. entiviral particles were produced with HEK-293T cells. A549 cells were infected with lentiviral particle solution supernatant and screened with puromycin. Then the migration of PAQR11 knockdown A549 cell was detected with erasion trace test and the invasion was observed with transwell chamber. The expression of EMT associated molecular was detected by real-time PCR.Results: Lentiviral vector for interfering PAQR11 was built. Stable PAQR11 knockdown A549 lung cancer cells were established. The knockdown of PAQR11 was tested by real-time PCR and Western blot. Cell migration and invasion was promoted in PAQR11 knockdown A549 lung cancer cells. PAQR11 knockdown could reduce the expression of E-Cadherin, but up-regulate the level of Snail1, Twist1 and N-Cadherin.Conclusion: Knockdown of PAQR11 could promote the invasion and metastasis of A549 lung cancer cells, and this effect may be relevant with reinforcing EMT.关键词
肺癌/孕激素和脂联素分子受体家族11/侵袭/转移/上皮间质转化Key words
Lung cancer/PAQR11/Invasion/Metastasis/EMT分类
医药卫生引用本文复制引用
李维娜,何飞,薛慧君,张敏,张晓军,曾召利..孕激素和脂联素分子受体11对A549肺癌细胞侵袭转移的影响研究[J].中国医学装备,2016,13(12):150-153,154,5.基金项目
陕西省自然科学基金面上资助项目(2015JM8470)“MiR-140-5p/PAQR11通路对肺癌的作用及机制” （2015JM8470）
