中国药业2016，Vol.25Issue(24)：16-18,19,4.
夏枯草配方颗粒高效液相色谱特征图谱研究
Study on the HPLC Specific Chromatogram of Prunella Vulgaris Formula Granules
胡莲 1周杰 1袁慧1
作者信息
- 1. 西南医科大学附属医院药学部，四川 泸州 646000
- 折叠
摘要
Abstract
Objective To establish the HPLC specific chromatogram of Prunella Vulgaris Formula Granules. Methods The specific chro-matograms were analyzed by Welch Materials AQ C18 ( 250 mm × 4. 6 mm, 5 μm ) and eluted with gradient acetonitrile and 0. 1% phos-phoric acid at a flow rate of 0. 8 mL∕min, detection wavelength of 280 nm, and sample volume of 10 μL. Results The specific chro-matograms of Prunella Vulgaris Formula Granules were obtained. There were 6 characteristic peaks, and among them were 1 min identi-fied by direct comparison with reference substances. The peak of rosmarinic acid is S peak. Calculate the relative retention time of each characteristic peaks and the S peak; the relative retention time should be 0. 260 ( peak 1 ) , 0. 404 ( peak 2 ) , 0. 540 ( peak 3 ) , 1. 000 ( peak 4 ) , 1. 360 ( peak 5 ) , 1. 836 ( peak 6 ) . Measure these 13 samples and the result was in accord with regulation. Conclusion The HPLC specific chromatograms is proved to be simple, stable and reproducible, and it can provide reference for the identification of Prunella Vulgaris Formula Granules.关键词
夏枯草/配方颗粒/特征图谱/高效液相色谱法Key words
Prunella vulgaris/formula granules/specific chromatograms/HPLC分类
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胡莲,周杰,袁慧..夏枯草配方颗粒高效液相色谱特征图谱研究[J].中国药业,2016,25(24):16-18,19,4.
