中国医药科学2016，Vol.6Issue(19)：36-39,43,5.
三维共培养模型下人脐静脉内皮细胞对鼻咽癌CNE2细胞侵袭、迁移及增殖能力的影响
Influence of human umbilical vein endothelial cells under the three-dimensional co culture model to invasion, migration and proliferation of nasopharyngeal carcinoma cell line CNE2
摘要
Abstract
ObjectiveTo explore the influence of human umbilical vein endothelial cells(HUVEC) under the three-dimensional co culture model to invasion, migration and proliferation of nasopharyngeal carcinoma cell line CNE2.Methods Transwell was used to give co culture of HUVEC cells and CNE2 cells.CCK-8 was used to compare the CNE2 cell proliferation.Cell counting method was used to compare the cell migration ability.Matrigel method was used to compare the invasive ability. ResultsUnder the co culture condition of CNE2 and HUVEC,the migration ability of co culture group was significantly higher than that of non co culture group(P<0.01).The invasive ability of the co culture group was significantly higher than that in the non co culture group(P<0.01).In the proliferation experiment,On the third day,fourth day and fifth day,the cell proliferation rate was significantly higher than that of non co culture co culture group(P<0.05).ConclusionUnder three dimensional co culture conditions,HUVEC can promote the invasion,migration and proliferation of CNE2 nasopharyngeal carcinoma cells.关键词
三维共培养模型/CNE2鼻咽癌细胞/人脐静脉内皮细胞/增殖/迁移/侵袭Key words
Three-dimensional co culture model/CNE2 nasopharyngeal carcinoma cell/Human umbilical vein endothelial cells/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
梁霞,高超,唐勇,刘翰楠,吴健勇,李宇虹,庞波,陈玉..三维共培养模型下人脐静脉内皮细胞对鼻咽癌CNE2细胞侵袭、迁移及增殖能力的影响[J].中国医药科学,2016,6(19):36-39,43,5.基金项目
国家自然科学基金资助项目（81560428）；广西自然科学基金资助项目（2011GXNSFC018020，2012GXNSFAA053163）；广西科学研究与技术开发计划项目（桂科攻1355005-3-11）；广西医疗卫生适宜技术研究与开发课题（S201301-06）；广西壮族自治区卫生厅科研课题（Z2011226）。 （）
