中国医药科学2016，Vol.6Issue(19)：48-51,4.
大麻素受体1基因多态性和肠易激综合征的关系
Relationship between cannabinoid receptor 1 gene polymorphism and irritable bowel syndrome
李洁 1黎钟燕 1辜金莲 1张龙1
作者信息
- 1. 广州市第一人民医院老年病科，广东广州 510180
- 折叠
摘要
Abstract
ObjectiveTo investigate the relationship between cannabinoid receptor 1 gene (CNR1) polymorphism and irritable bowel syndrome.Methods60 cases of patients with irritable bowel syndrome cured in GI medicine and geriatrics department of our hospital from September 2014 to September 2016 and 60 cases of healthy patients were selected as the research objects.The 60 cases of patient with irritable bowel syndrome were selected as observation group,the 60 cases of patients of health examination during the same period were selected as control group.PCR technology were used to amplify the genomic DNA in serum samples,and its sequence was determined. Common alleles and genotypes of the two groups were compared,and the relationship between CNR1 polymorphism and irritable bowel syndrome were analyzed.ResultsThe genomic DNA amplification of the target fragment sequence determination revealed that there were 8 CNR1 alleles and 20 genotypes.The research object in the highest frequency of the allele was AAT13,the most common genotype was 13/13.The frequencies of AAT13 and 13/13 genotypes in the observation group were 51.67% and 33.33% respectively,which were significantly higher than the control group(33.33%,16.67%). Some preliminary results,with division of allele AAT10 boundaries,the frequency of allele AAT≥10 (96.67%) and genotype frequency≥10/≥10(93.33%)in observation group were significantly higher than that in control group with allele AAT≥10(83.33%)and genotype frequency≥10/≥10(80%),with statistically significant(P<0.05). ConclusionThe patients with irritable bowel syndrome CNR1 gene allele frequency is the highest in AAT13,and the most widely is genotype 13/13.CNR1 genotype polymorphism and irritable bowel syndrome occurred in the presence of some contact with≥10 alleles and genotypes≥10/≥10 people are more likely to suffer from irritable bowel syndrome,and the irritable bowel syndrome early diagnosis has important clinical significance.关键词
大麻素受体1基因/基因多态性/等位基因/基因型/肠易激综合征Key words
Cannabinoid receptor 1 gene/Gene polymorphism/Allele/Genotype/Irritable bowel syndrome分类
医药卫生引用本文复制引用
李洁,黎钟燕,辜金莲,张龙..大麻素受体1基因多态性和肠易激综合征的关系[J].中国医药科学,2016,6(19):48-51,4.
