中国医药科学2016，Vol.6Issue(19)：64-67,134,5.
毒热清颗粒的质量标准研究
Quality Standard Study of dureqing granule
摘要
Abstract
ObjectiveTo establish quality standard of new preparations of dureqing granule.Methods Forsythia,Violet,Licorice in dureqing granule were identified by TLC.The contents of baicalin and chlorogenic acid were determined by HPLC,which was performed on an Agilent ZORBAX XDB-C18 column (4.6mm×250mm,0.45μm) with a mobile phase methanol-0.4% phosphoric acid (gradient elution),at a flow rate of 1.0mL/min.The detection wavelength was 316nm,with column temperature 25℃.ResultsChromatographic characteristics of qualitative identification were evident.The linear range of baicalin was 0.4764~2.382μg (r=0.9999) and the average recovery rate was 98.8% (RSD=1.5%).The linear range of chlorogenic acid was 0.4216~2.108μg (r=0.9999) and the average recovery rate was 99.1% (RSD=1.1%). RSDs of precision, stability and reproducibility tests were lower than 2.0%(n=6). Conclusion The method of qualitative and quantitative analysis is accurate,feasible and suitable for effective quality control of dureqing granule.关键词
毒热清颗粒/黄芩苷/绿原酸/薄层色谱/高效液相色谱Key words
HPLC/TLC/Baicalin/Chlorogenic acid/Dureqing granule分类
医药卫生引用本文复制引用
郑弘,秦会珍,施钧瀚,孟祥乐..毒热清颗粒的质量标准研究[J].中国医药科学,2016,6(19):64-67,134,5.基金项目
国家自然科学基金-河南省人民政府人才培养联合基金（U1304824）；中国博士后科学基金特别资助（2015T80772）；中国博士后科学基金面上资助项目（2015M582190）；河南省博士后基金项目（2014-75）；河南省济源市科技攻关项目（15013034）；河南省科技厅“三区”人才计划 （）
