中国医药科学2016，Vol.6Issue(20)：7-10,63,5.
两种检测方法对71例非综合征型耳聋患者基因检测结果的对比分析
Contrast analysis of test results of two kinds of detection methods in 71 cases of non-syndromic deafness patients
摘要
Abstract
Objective Using two different of methods to test 71 cases of non-syndromic deafness patients about the deafness gene, to provide more meaningful screening for clinic, to improve the deafness gene detection rate. Methods71 cases of non-syndromic deafness patients from July 2014 to December 2015 were selectrd as the study objects. Chip hybridization and multiple fluorescent PCR two different methods were used to test the 71 cases of non-comprehensive deafness patients about deafness gene, and SPSS17.0 software was used for the data analysis.Results There were significant diffience between the two kinds of methods for 71 cases of non-syndromic deafness patients (P<0.05). The deafness gene detection rate of chip hybridization method was 14.08% (10/71) , and the deafness gene detection rate of multiple fluorescent polymerase chain reaction (PCR) method was 30.98 (22/71) . Deafness gene's mutation site of the two methods was different, which led to a significant difference in detection rate. Conclusion Compared with chip detection method , increasing testing site in multiple fluorescent polymerase chain reaction ( PCR ) is meaningful for improving the clinical deafness gene screening rate .关键词
耳聋/基因/突变/检出率Key words
Deafness/Genes/Mutation/Detection rate分类
医药卫生引用本文复制引用
韩锐,李林格,段玲,夏燕,陈俞..两种检测方法对71例非综合征型耳聋患者基因检测结果的对比分析[J].中国医药科学,2016,6(20):7-10,63,5.基金项目
新疆维吾尔自治区自然科学基金资助项目（2013211A102）。 （）
