滨州医学院学报2016，Vol.39Issue(6)：404-407,444,5.
PCOS大鼠卵巢中 kisspeptin／kiss1r 系统的表达变化及对卵泡发育的影响
The role of kisspeptin/kiss1r system on follicular development disorder in the ovaries of polycystic ovary syndrome rats
史学燕 1骆倩倩 2王立桐 3蔡恒 4张洪芹1
作者信息
- 1. 滨州医学院基础医学院组织学与胚胎学教研室烟台 264003
- 2. 东营市人民医院血管外科
- 3. 滨州医学院实验教学管理中心形态学实验室
- 4. 滨州医学院临床医学院
- 折叠
摘要
Abstract
Objective To explore the role of kisspeptin/kiss1r system on follicular developmental disorder in the ovaries of polycystic ovary syndrome (PCOS) rats .Methods The PCOS rat models were constructed by Letrozole with intraperitoneal in‐jection .Serum hormone levels and morphological changes of ovary were examined by ELISA and H‐E staining ,respectively . The expression of kisspeptin/kiss1r system and FSHR in experimental rat ovaries was detected through immunohistochemistry . Results Compared with the normal group ,body weight of the PCOS group increased significantly from the beginning of the tenth day after filling the stomach .Simultaneously ,LH/FSH ratio was considerably increased ,which lead to hyperandrogenism . Small follicles and atresia follicles were occupied the ovarian cortex of PCOS and most of the follicles showed cystic dilatation whose cell layers were significantly reduced .FSHR and kisspeptin/kiss1r were expressed in the cytoplasm of ovaries ,and kisspeptin/kiss1r was mainly expressed in granulosa cells ,membrane cells and corpora luteum ,whereas FSHR was expressed only in granulosa cells .The expression of kisspeptin/kiss1r and FSHR in the PCOS group was significantly decreased .Conclu‐sion Kisspeptin/kiss1r system expressed in the ovaries may play an important role in the process of follicular developmental disorder ,which provides a novel idea for clinical treatment and basic research of PCOS .关键词
kisspeptins/多囊卵巢综合征/卵泡发育Key words
kisspeptins/polycystic ovary syndrome/follicular development分类
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史学燕,骆倩倩,王立桐,蔡恒,张洪芹..PCOS大鼠卵巢中 kisspeptin／kiss1r 系统的表达变化及对卵泡发育的影响[J].滨州医学院学报,2016,39(6):404-407,444,5.
