江汉大学学报（自然科学版）2016，Vol.44Issue(6)：496-503,8.DOI:10.16389/j.cnki.cn42-1737/n.2016.06.003
菜豆鲜荚中菜豆凝集素的提取与分离纯化工艺研究
Extraction,Separation and Purification of Phaseolus vulgaris Agglutinin(PHA)from Common Bean Fresh Pods
摘要
Abstract
In this paper ,the processes of extraction ,separation and purification of Phaseolus vulgaris agglutinin (PHA) from the common bean "Bean Saint esprit a oeil rouge" fresh pods were optimized. The effects of extracting agent ,material-liquid ratio ,pH and extracting time on PHA were studied , and the combined method of ammonium sulfate precipitation , DEAE- 52 anion exchange chromatography and Superdex-200 gel filtration chromatography for extracting ,isolating and purifing the PHA from fresh pods of common bean has been established. The main results were as follow:1)The optimized technical parameters for common bean extraction process were material-liquid ratio 1∶25 , pH 6-8 ,extracting time 8 h ,PBS as extracting agent;2)The high purity PHA was obtained with ammonium sulfate precipitation ,dialysis ,DEAE-52 anion exchange chromatography ,concentrated by ultrafiltration and Superdex-200 gel filtration chromatography. The obtained PHA shows one band with electrophoresis test and has blood clotting activity.关键词
菜豆/凝集素/分离纯化/血凝活性Key words
common bean/lectin/separation and purification/hemagglutination activity分类
医药卫生引用本文复制引用
陈高,王红波,郭瑞,代祥德,刘彤,陈禅友..菜豆鲜荚中菜豆凝集素的提取与分离纯化工艺研究[J].江汉大学学报（自然科学版）,2016,44(6):496-503,8.基金项目
农业部948项目（2011-G1-17）；湖北省科技平台项目（鄂科技通［2011］第101号）；湖北省资源中心项目（2015BCE091）；武汉市科技攻关项目 （）
