烟草科技2016，Vol.49Issue(12)：8-12,5.DOI:10.16135/j.issn1002-0861.2016.0267
授粉时期和套袋方式对烟草母本起源单倍体鉴定效率的影响
Effects of pollination time and bagging means on identification of tobacco maternal haploids
摘要
Abstract
Maternal haploid (MH) was obtained by the interspecific hybridization method. In this system, Nicotiana tabacum was hybridized as a female with Nicotiana africana due to parthenogenesis. In order to overcome the problems such as low induction rate of maternal haploid and difficult identification of maternal haploid from aneuploid interspecific hybrids in breeding practice, flow cytometry (FCM) was adopted to identify the maternal haploids of hybrid progenies produced by different bagging means at different pollination time. The results showed that maternal haploids were accurately and quickly identified by FCM at seedling stage and eliminated the disturbances from diploids and aneuploid interspecific hybrids. The survival rates of non-haploid seedlings bagged with paper tube and common paper bag were 0.24% and 1.56%,respectively. The identification efficiency of paper tube bagging means for haploid was higher. The suitable time for hybridization was identified as when the difference between sepal length and corolla length (SCD) was 2.5 cm,which was beneficial to the induction of maternal haploids. Therefore,the better way for obtaining maternal haploid was pollination at SCD of 2.5 cm,adopting paper tube bagging means and analyzing progeny ploidy with FCM.关键词
普通烟草(Nicotiana tabacum)/非洲烟草(N. africana)/流式细胞术/授粉时期/套袋方法Key words
Nicotiana tabacum/Nicotiana africana/Flow cytometry(FCM)/Pollination time/Bagging means分类
农业科技引用本文复制引用
曾建敏,焦芳婵,童治军,肖炳光,李永平,陈学军..授粉时期和套袋方式对烟草母本起源单倍体鉴定效率的影响[J].烟草科技,2016,49(12):8-12,5.基金项目
中国烟草总公司科技项目“新引进野生烟草植物学性状调查及主要病害抗病特性挖掘研究”（110201302005）、“中美合作选育抗黑胫病优质烤烟新品种”（110201402002）；中国烟草总公司云南省公司科技项目“中美烤烟新品种合作选育”（2014YN04）、“新引进野生烟草植物学性状调查及主要病害抗病特性挖掘研究”（2013YN09）。 （）
