烟草科技2016，Vol.49Issue(12)：13-20,8.DOI:10.16135/j.issn1002-0861.2016.0118
洛阳地区烟草根际土壤中多粘类芽孢杆菌的分离与鉴定
Isolation and identification of Paenibacillus polymyxa from rhizosphere soil of tobacco plantation in Luoyang
摘要
Abstract
To isolate bacteria strains which possess antagonistic activities against soil-borne pathogenic fungi of tobacco from tobacco rhizosphere soil,dilution plating method and dual culture method were adopted to isolate and screen antagonistic bacteria. The bacteria morphological features were observed, and in addition physiological and biochemical experiments and 16S rDNA sequence analysis were used to identify antagonistic strains. A total of 180 bacterial isolates were obtained from 30 rhizosphere soil samples by separation and purification,in which 49 strains had antagonistic function against soil-borne pathogenic fungi. Strain TS681, TS682 and TS683 were strongly antagonistic towards three kinds of pathogenic fungi,and its antagonistic rate to Pythium aphanidermatum,Phytophthora parasitica and Furasium oxysporum reached above 51.43%,68.75%and 60.00%,respectively. Combined with morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence analysis, the above three strains were identified as Paenibacillus polymyxa.关键词
烟草/土传病原真菌/拮抗细菌/多粘类芽孢杆菌Key words
Tobacco/Soil-borne pathogenic fungus/Antagonistic bacterium/Paenibacillus polymyxa分类
农业科技引用本文复制引用
宋喜乐,赵世民,赵云波,江凯,康业斌..洛阳地区烟草根际土壤中多粘类芽孢杆菌的分离与鉴定[J].烟草科技,2016,49(12):13-20,8.基金项目
河南省烟草公司科技项目“洛阳市烟草土传真菌病害种类及微生态治理措施研究与应用”（2012M10）；洛阳市烟草公司科技项目“洛阳市烟草腐霉菌多样性及其拮抗微生物研究”（2014M06）。 （）
