北华大学学报（自然科学版）2017，Vol.18Issue(1)：38-42,5.DOI:10.11713/j.issn.1009-4822.2017.01.008
林下参花挥发油化学成分及其抗肿瘤细胞增殖活性研究
Chemical Composition and Anti-Proliferations of Essential Oil from Flowers of Ginseng under Forest in Tumor Cells
摘要
Abstract
Objective To investigate the chemical composition and anti-proliferations of essential oil from flowers of ginseng under forest ( GUF ) . Method Essential oil of GUF flowers ( GUFFEO ) was extracted by hydrodistillation and analyzed by GC-MS respectively. The cytotoxicities of GUFFEO on mouse fibroblasts ( NIH-3T3 ) and the effects of GUFFEO on the proliferation of human lung adenocarcinoma ( A549 ) , human cervical cancer (HeLa),human gastric cancer (SGC7901),mouse melanoma (B16) and adrenal cortical (Y1) cell lines were assayed by MTT. Results 85 components of GUFFEO were identified by GC-MS. It contained a complex mixture consisting of aliphatic,terpenoid and aromatic compounds. The major constituents were palmitic acid,linoleic acid and tridecanoic acid. There was no cytotoxicity of GUFFEO on the 3T3 cell lines with the concentration under 400 μg/mL. GUFFEO exhibited significant inhibition of Y1, HeLa, SGC-7901 and B16 cancer cells growth,and no effect on A549 cell proliferation. Conclusion The chemical composition of essential oil from flowers of ginseng under forest mainly contained aliphatic and terpenoid compounds,and it was found that it can inhibit the proliferations of many cancer cell lines.关键词
林下参花/挥发油/肿瘤细胞/增殖Key words
flowers of ginseng under forest/essential oil/tumor cells/proliferations分类
医药卫生引用本文复制引用
徐晓浩,孙立伟,姜锐..林下参花挥发油化学成分及其抗肿瘤细胞增殖活性研究[J].北华大学学报（自然科学版）,2017,18(1):38-42,5.基金项目
国家自然科学基金项目(81641138) （81641138）
吉林省教育厅科学技术研究项目(2013183) （2013183）
吉林市科技发展计划项目(20156410) （20156410）
