北京中医药大学学报2017，Vol.40Issue(1)：20-26,7.DOI:10.3969/j.issn.1006-2157.2017.01.005
红芪多糖对db／db小鼠糖尿病心肌病TGF-β1／Smads信号通路影响的实验研究
Effect of hedysarum polybotrys polysacchcaide on TGF-β1/Smads signa-ling pathway in db/db mice with diabetic cardiomyopathy
摘要
Abstract
Objective To explore the effect of hedysarum polybotrys polysacchcaide (HPS)on myocar-dial fibrosis of db /db mice with diabetic cardiomyopathy (DCM)by taking TGF-β1 /Smads signaling pathway as the index.Methods Altogether 60 six-week-old male db /db mice were randomly divided in-to five groups:HPS high-,mid-,low-dose group,rosiglitazone group and model group,additional 12 non transgenic db /m male mice with the same background were assigned into the normal group.The mice ex-cept for those of the normal group were intragatrically administered (i.g.)corresponding medicines for consecutive 8 weeks.Before treatment and every two weeks during the period of i.g.drugs,the body weight and blood glucose were detected.At the end of Week 8,HE staining was used to observe the pathological changes of left ventricular myocardial fibers,Masson staining was used to observe the degree of left ventricular myocardial collagen fibrosis,and Western blot,RT-PCR were used to measure the pro-tein and mRNA expressions of TGF-β1 and Smad2,Smad3 in cardiac muscle tissue.Results Eight weeks after treatment,the blood glucose of rats in HPS high-,mid-dose group,and rosiglitazone group decreased significantly compared with model group (P <0.05).The sections of HE staining showed that the model rats’myocardial cells structure became unclear and some of them were swollen and deformed obviously,even the nucleus disappeared.The sections of Masson staining showed that bright green colla-gen fibers were heaped up between myocardial cells and around blood vessels of the model rats.The pro-tein and mRNA expressions of TGF-β1 ,Smad2 and Smad3 had no significant difference between model group and HPS low-dose group (P >0.05),but the other groups decreased significantly compared with the model group (P <0.05),especially the HPS high-dose group or rosiglitazone group was superior to mid-dose group (P <0.05).Conclusion The effect of HPS against myocardial fibrosis in db /db mice with DCMto slow down the progression of DCMwas proved,and its potential mechanism may be related to inhibitation of TGF-β1 /Smads signaling pathway.关键词
糖尿病心肌病/红芪多糖/TGF-β1/Smads信号通路/db/db小鼠Key words
diabetic cardiomyopathy/hedysarum polybotrys polysacchcaide/TGF-β1 /Smads signaling pathway/db /db mice分类
医药卫生引用本文复制引用
金智生,王东旭,张花治,和彩玲,南向萍,王倩,楚惠媛..红芪多糖对db／db小鼠糖尿病心肌病TGF-β1／Smads信号通路影响的实验研究[J].北京中医药大学学报,2017,40(1):20-26,7.基金项目
国家自然科学基金项目 （）
