东南大学学报（英文版）2016，Vol.32Issue(4)：496-501,6.DOI:10.3969/j.issn.1003-7985.2016.04.017
高浓度氯化钾盐溶液中poly（dT）20在α溶血素纳米孔内的电泳动力学
Electrophoresis of poly(dT) 20 through α-hemolysin nanopore in high concentration potassium chloride solution
摘要
Abstract
Experiments of poly ( dT ) 20 electrophoresis throughα-hemolysin nanopores were performed to unveil the electrophoretic transport mechanism of DNA through nanopores in high concentration potassium chloride solution. It is found that there are two obvious current blockades induced by poly ( dT ) 20 translocation and collision events. Both blockade currents increase linearly with the applied bias voltage. However, the normalized blockade currents are almost kept the same although variable bias voltages are applied. The collision time of poly( dT) 20 in the luminal site of the pore remains constant for different voltages. The translocation speed of poly ( dT ) 20 through the nanopore decreases with the increase of bias voltage. It is because as the potential increases, the drag force on the homopolymer helps it to crumple into a cluster much easier due to the poor stacking of thymine residues compared with homopolymers consisting of other nucleotides. Molecular dynamics simulations further confirm the experimental results. Increasing the applied bias voltage can slow down the translocation velocity of the flexible poly( dT ) 20 , which favors increasing the precision of single molecule detection by using nanopores.关键词
纳米孔/过孔速度/离子电流/电泳Key words
nanopore/transport speed/ionic current/electro-phoresis分类
机械制造引用本文复制引用
司伟,杨浩杰,纪安平,李堃,沙菁,刘磊,陈云飞..高浓度氯化钾盐溶液中poly（dT）20在α溶血素纳米孔内的电泳动力学[J].东南大学学报（英文版）,2016,32(4):496-501,6.基金项目
The National Natural Science Foundation of China ( No.51435003,51375092), Research Program of Chongqing Municipal Education Commission ( No. KJ1401030), the Research &Innovation Program for Graduate Student in Universities of Jiangsu Province ( No. KYLX_0100), the Scientific Research Foundation of Graduate School of Southeast University ( No. YBJJ1540) （ No.51435003,51375092）
