福建农业学报2016，Vol.31Issue(10)：1015-1019,5.DOI:10.19303/j.issn.1008-0384.2016.10.002
番鸭呼肠孤病毒σC基因重组腺病毒载体的构建和鉴定
Construction and Certification of Recombinant Adenovirus Vector forσC Gene in Muscovy Duck Reovirus
摘要
Abstract
TheσC gene of muscovy duck reovirus (MDRV)was amplified by RT-PCR and cloned into pShuttle-CMV plasmid after the amplicon was digested by XhoI+Hind III enzyme.Sequencing analysis confirmed the gene to beσC.Subsequently,the recombinant plasmid pShuttle-CMV-σC and the adenovirus backbone vector,pAdEasy-1 , were co-transfected into 293 cells to construct a recombinant adenovirus.The obtained recombinant adenovirus pAd-σC was positively identified by PCR and RT-PCR.The information obtained could be used for genetic engineering a vaccine against MDRV.关键词
番鸭呼肠孤病毒/σC基因/重组腺病毒载体Key words
muscovy duck reovirus/σC gene/recombinant adenovirus vector分类
农业科技引用本文复制引用
林锋强,程晓霞,王劭,朱小丽,王锦祥,陈仕龙,陈少莺..番鸭呼肠孤病毒σC基因重组腺病毒载体的构建和鉴定[J].福建农业学报,2016,31(10):1015-1019,5.基金项目
国家自然科学基金项目(31172334) （31172334）
福建省自然科学基金项目(2016J01137) （2016J01137）
