福建农业学报2016,Vol.31Issue(10):1020-1023,4.DOI:10.19303/j.issn.1008-0384.2016.10.003
禽腺病毒4型的分离与初步鉴定
Isolation and Preliminary Identification of Avian Adenovirus Serotype 4
摘要
Abstract
A viral strain,designated as FJ1674,was isolated from dead chickens that showed hydropericardium syndrome. The gene fragment of the strain,which lacked the hemagglutination activity,could be amplified by the specific primers of avian adenovirus serotype 4 against those of the viruses of avian influenza,newcastle disease,avian infectious bronchitis,avian encephalomyelitis, avian leukemia, infectious bursa, avian metapneumo, and inclusion body hepatitis.Thus,FJ1674 was believed to belong to avian adenovirus serotype 4.The sequencing analysis revealed its high homology (99.4%-100%)between FJ1674 and its counterpart in GenBank.The phylogenetic analysis also showed that FJ1674 was close to viruses of the same serotype,and could be subdivided into the same isolate cluster.关键词
鸡心包积液综合征/禽腺病毒4 型/分离鉴定Key words
chicken hydropericardium syndrome/avian adenovirus serotype 4/isolation and identification分类
农业科技引用本文复制引用
陈珍,林羽,施少华,黄瑜,韩跃松,陈翠腾,刘荣昌,蔡国漳,朱春华,刘斌琼..禽腺病毒4型的分离与初步鉴定[J].福建农业学报,2016,31(10):1020-1023,4.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项(2016R1022-1、2016R1022-9) (2016R1022-1、2016R1022-9)
福建省畜禽疫病防控技术重大研发平台子专题项目(2014N2003-8) (2014N2003-8)
福建省扶贫项目(2016) (2016)