福建农业学报2016，Vol.31Issue(10)：1075-1079,5.DOI:10.19303/j.issn.1008-0384.2016.10.014
铁皮石斛茎段原球茎的诱导、分化与植株再生
Protocorm Induction/Differentiation and Plantlet Regeneration of Stems from Dendrobium officinale Kimura et Migo
摘要
Abstract
To establish an effective and rapid propagation method for Dendrobiumofficinale,sterile stem cuttings from the plant were used as the explants for the study.Effects of cultivation media,growth regulators,sucrose concentrations,and additives on the protocorm induction,proliferation and differentiation,as well as the rooting of the plantlets were investigated in an orthogonal experiment.The results showed that the optimal medium for the protocorm induction consisted of 1/2MS+6-BA4 mg·L-1+NAA0.1 mg·L-1;that for the proliferation,MS+30 g·L-1;that for the differentiation,MS+10 g·L-1 sucrose +10% potato slurry;and,that for the rooting,1/2MS+NAA1 mg·L-1 +10% potato slurry+10% banana slurry.The method appeared to enable a successful regeneration of D.officinale plantlets through tissue culture propagation.关键词
铁皮石斛/原球茎/正交试验/植株再生Key words
Dendrobiumofficinale/protocorm/orthogonal experiment/plantlet regeneration分类
农业科技引用本文复制引用
林江波,王伟英,李海明,吴水金,邹晖,李跃森,戴艺民..铁皮石斛茎段原球茎的诱导、分化与植株再生[J].福建农业学报,2016,31(10):1075-1079,5.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项(2014R1013-3) （2014R1013-3）
